Characterization of a Bombyx mori nucleopolyhedrovirus mutant lacking both fp25K and p35
Characterization of a Bombyx mori nucleopolyhedrovirus mutant lacking both fp25K and p35
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DOI：
10.1007/s11262-010-0492-5
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发表时间：
2010-05
期刊：
影响因子：
1.6
通讯作者：
Tadashi Nakanishi;T. Shimada;S. Katsuma
中科院分区：
文献类型：
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作者：
Tadashi Nakanishi;T. Shimada;S. Katsuma
Recent studies have shown that dual mutations infp25Kandp35ofAutographa californicanucleopolyhedrovirus (AcMNPV) result in a typical apoptotic infection onTrichoplusia nicells, suggesting the involvement of FP25K on NPV-induced apoptosis. To examine the effect offp25Kdeletion onBombyx moriNPV (BmNPV)-induced apoptosis, we generated a BmNPV mutant, fp-p35D, in which bothfp25Kandp35genes are deleted from the genome, and compared its phenotype with wild-type (T3),fp25K-deleted (fp-null), andp35-deleted (p35D) BmNPVs. In BmN cells, p35D, but not T3 or fp-null, caused apoptosis with caspase-3 activation. Infection with fp-p35D also resulted in caspase-3 activation, but the level was comparable to that of p35D. Also, we did not observe any apoptotic responses in hemocytes from larvae infected with p35D or fp-p35D. These results indicate that unlike AcMNPV, deletion offp25Kdoes not affect the pathway of p35D-induced apoptosis of BmN cells andB. morilarvae.
