Bone formation by three-dimensional stromal osteoblast culture in biodegradable polymer scaffolds.
Bone formation by three-dimensional stromal osteoblast culture in biodegradable polymer scaffolds.
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DOI：
10.1002/(sici)1097-4636(199707)36:1
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发表时间：
1997-07
期刊：
影响因子：
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通讯作者：
S. L. Ishaug;G. Crane;Michael J. Miller;A. Yasko;M. Yaszemski;A. Mikos
中科院分区：
文献类型：
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作者：
S. L. Ishaug;G. Crane;Michael J. Miller;A. Yasko;M. Yaszemski;A. Mikos
通过在三维(3-D)可生物降解的聚(DL-乳酸-乙醇酸)泡沫中培养基质成骨细胞来研究骨的形成。在56天的培养期间，检测了三种聚合物泡沫孔径，范围从150-300微米，300-500微米，和500-710微米，以及两种不同的细胞种植密度，6.83×10(5)个/cm2和22.1×10(5)个/cm2。聚合物泡沫支持种子成骨细胞的增殖及其分化功能，表现为高碱性磷酸酶活性和细胞沉积矿化基质。随着时间的推移，所有聚合物泡沫的细胞数量、碱性磷酸酶活性和矿物沉积都显著增加。将6.83×10(5)个/cm2的成骨泡沫材料接种到孔径为300~500微米的泡沫材料上，培养1d后细胞密度为4.63×10(5)个/cm2，第7天和第28天的碱性磷酸酶活性分别为4.28×10(-7)和2.91×10(-6)个/cm2，第56天细胞密度增加到18.7×10(5)个/cm2。对于相同的结构，矿化基质从泡沫的顶部表面达到的最大渗透深度为240µm，单位横截面面积的矿化组织体积为0.083 mm。种植密度是构建物的一个重要参数，但测试范围内的孔径大小不影响细胞增殖或功能。本研究提示聚α-羟基酯泡沫塑料作为支架材料用于自体成骨细胞移植再生骨组织的可行性。
Bone formation was investigated in vitro by culturing stromal osteoblasts in three-dimensional (3-D), biodegradable poly(DL-lactic-co-glycolic acid) foams. Three polymer foam pore sizes, ranging from 150-300, 300-500, and 500-710 microns, and two different cell seeding densities, 6.83 x 10(5) cells/cm2 and 22.1 x 10(5) cells/cm2, were examined over a 56-day culture period. The polymer foams supported the proliferation of seeded osteoblasts as well as their differentiated function, as demonstrated by high alkaline phosphatase activity and deposition of a mineralized matrix by the cells. Cell number, alkaline phosphatase activity, and mineral deposition increased significantly over time for all the polymer foams. Osteoblast foam constructs created by seeding 6.83 x 10(5) cells/cm2 on foams with 300-500 microns pores resulted in a cell density of 4.63 x 10(5) cells/cm2 after 1 day in culture; they had alkaline phosphatase activities of 4.28 x 10(-7) and 2.91 x 10(-6) mumol/cell/min on Days 7 and 28, respectively; and they had a cell density that increased to 18.7 x 10(5) cells/cm2 by Day 56. For the same constructs, the mineralized matrix reached a maximum penetration depth of 240 microns from the top surface of the foam and a value of 0.083 mm for mineralized tissue volume per unit of cross sectional area. Seeding density was an important parameter for the constructs, but pore size over the range tested did not affect cell proliferation or function. This study suggests the feasibility of using poly(alpha-hydroxy ester) foams as scaffolding materials for the transplantation of autogenous osteoblasts to regenerate bone tissue.