SCRAMBLED ACTIN I GENE IN THE MICRONUCLEUS OF OXYTRICHA-NOVA
SCRAMBLED ACTIN I GENE IN THE MICRONUCLEUS OF OXYTRICHA-NOVA
复制标题
DOI：
10.1002/dvg.1020130111
复制
发表时间：
1992-01-01
期刊：
影响因子：
--
通讯作者：
GRESLIN, AF
中科院分区：
文献类型：
--
作者：
PRESCOTT, DM;GRESLIN, AF
在hypotrichous纤毛虫Oxytricha nova中，从编码肌动蛋白I的微核基因组克隆的前体基因由高度无序的脱氧核苷酸序列块组成。我们提出并详细说明了一个重组模型，解释了如何肌动蛋白I基因可能是unscrambled在细胞交配后的大核发育。该模型在先前的出版物中描述（Greslin等人：Proc Natl Acad Sci USA 86：6264 - 6268，1989）。在这里，我们展示了支持该模型的数据，这些数据在早期的出版物中有所描述。数据显示，加扰不是克隆的产物。他们反对在微核中存在肌动蛋白I基因的未加扰拷贝，这意味着未加扰必须是大核发育的一部分。最后，证明了O. trifallax的排列模式与O. novo.
In the hypotrichous ciliate Oxytricha nova the cloned precursor gene from the micronuclear genome that encodes actin I is composed of highly disordered blocks of deoxynucleotide sequences. We present and illustrate in detail a recombination model that explains how the actin I gene may be unscrambled during macronuclear development after cell mating. The model was described in a previous publication (Greslin et al.: Proc Natl Acad Sci USA 86:6264-6268, 1989). Here we show the data, described in the earlier publication, that support the model. The data show that scrambling is not an artifact of cloning. They rule against the presence of an unscrambled copy of the actin I gene in the micronucleus, which means that unscrambling must be a part of macronuclear development. Finally, the data prove that the actin I gene in O. trifallax is scrambled in a pattern that resembles the pattern in O. novo.