Microarray analysis of differentially expressed microRNAs in non-regressed and regressed bovine corpus luteum tissue; microRNA-378 may suppress luteal cell apoptosis by targeting the interferon gamma receptor 1 gene
Microarray analysis of differentially expressed microRNAs in non-regressed and regressed bovine corpus luteum tissue; microRNA-378 may suppress luteal cell apoptosis by targeting the interferon gamma receptor 1 gene
复制标题
DOI：
10.1007/s13353-011-0055-z
复制
发表时间：
2011-11-01
影响因子：
2.4
通讯作者：
Zhang, Jiabao
中科院分区：
文献类型：
--
作者：
Ma, Tenghe;Jiang, Hao;Zhang, Jiabao
MicroRNAs(MiRNAs)是一种小的非编码内源性RNA分子，以序列依赖的方式下调靶基因的表达。最近的研究表明miRNAs机械地参与了哺乳动物黄体(CL)的调节。然而，很少有研究描述不同的miRNA在牛非退化和退化CL中的表达模式。本研究利用miRNA微阵列技术研究了不同的miRNA在牛CL中的表达模式。在13个差异表达的miRNAs中，7个在非退化型CL中优先表达，6个在退化型CL中高表达。应用实时荧光定量RT-PCR技术对芯片结果进行验证。已知与细胞凋亡有关的miR-378miRNA在非退化性CL中上调8.54倍(P<；0.01)，并预测可能在黄体细胞凋亡中发挥作用的干扰素伽玛受体1(IFNGR1)基因是mir-378的靶点。Mir-378实时荧光定量RT-PCR和蛋白质印迹分析结果表明，mir-378能降低人结缔组织中IFNGR1蛋白的表达，但不影响IFNGR1mRNA的表达。综上所述，我们的数据支持miRNA在牛CL细胞凋亡中的直接作用。
MicroRNAs (miRNAs) are small non-coding endogenous RNA molecules that down-regulate the expression of target genes in a sequence-dependent manner. Recent studies indicated that miRNAs are mechanistically involved in the regulation of the mammalian corpus luteum (CL). However, few studies have profiled the different miRNA expression patterns in bovine non-regressed and regressed CL. In this study, miRNA microarray was employed to investigate the different miRNA expression patterns in bovine CL. Among the 13 differentially expressed miRNAs, seven were preferentially expressed in non-regressed CL, while six miRNAs were more highly expressed in regressed CL. Real-time RT-PCR was used to validate the microarray results. Mir-378 miRNA, known to be associated with apoptosis, was 8.54-fold (P < 0.01) up-regulated in non-regressed CL, and the interferon gamma receptor 1 (IFNGR1) gene, which potentially plays a role in apoptosis of the luteal cell, was predicted to be the target of mir-378. The results of real-time RT-PCR of mir-378 and western blot analysis of the IFNGR1 protein at different stages of CL development showed that mir-378 decreased the expression of IFNGR1 protein but not IFNGR1 mRNA. Taken together, our data support a direct role for miRNA in apoptosis of bovine CL.