Functional bacterial and archaeal community structures of major trophic groups in a full-scale anaerobic sludge digester
Functional bacterial and archaeal community structures of major trophic groups in a full-scale anaerobic sludge digester
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DOI：
10.1016/j.watres.2006.12.036
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发表时间：
2007-04-01
期刊：
影响因子：
12.8
通讯作者：
Okabe, Satoshi
中科院分区：
文献类型：
--
作者：
Ariesyady, Herto Dwi;Ito, Tsukasa;Okabe, Satoshi
采用全周期16 SrRNA技术，结合显微放射自显影（MAR）-荧光原位杂交（FISH）技术和显微操作技术，研究了厌氧污泥消化池中功能菌和微生物群落结构。基于16 SrRNA克隆文库，用一套完整的16 S和23 SrRNA靶向探针进行FISH分析，结果表明，以探针HGC 69 A为代表的革兰氏阳性菌与放线菌杂交，（占4 '，6-二脒基-2-苯基吲哚（DAPI）染色的细胞）和探针LGC 354杂交的厚壁菌门（3.8 +/- 0.8%）是主要的系统发育细菌门，其次是拟杆菌门（4.0 +/- 1.2%）和绿杆菌门（3.7 +/- 0.8%）。探针MX 825杂交的甲烷菌（7.6 +/- 0.8%）是最丰富的古菌群，其次是甲烷微生物目（2.8 +/- 0.6%）和甲烷杆菌科（2.7 +/- 0.4%）。利用MAR-FISH技术对主要营养类群的功能群落结构（多样性和相对丰度）进行了定量分析。结果表明，葡萄糖降解菌群具有较高的丰度（约1000）。10.6+/- 4.9%的总DAPI染色的细胞）和多样性（至少七个系统发育组）相比，脂肪酸利用微生物群落，这是更专门的一些系统发育组。尽管β-变形菌属占优势，但Chloroflexi，Smithella，Syntrophomonas和Methanosaeta类群的成员占[C-14]葡萄糖-，[C-14]丙酸-，[C-14]丁酸-和[C-14]乙酸-利用微生物群落的主导地位，分别占27.7 +/-4.3%，29.6 +/-7.0%，分别为34.5 ± 7.6%和18.2 ± 9.5%。尽管丰度很低（约。1%），迄今未知的代谢功能的螺旋体和候选门的TM 7以及增效被发现是葡萄糖和乙酸的利用分别。(c)2007爱思唯尔有限公司保留所有权利。
Functional Bacteria and Archaea community structures of a full-scale anaerobic sludge digester were investigated by using a full-cycle 16S rRNA approach followed by microautoradiography (MAR)-fluorescent in situ hybridization (FISH) technique and micromanipulation. FISH analysis with a comprehensive set of 16S and 23S rRNA-targeted oligonucleoticle probes based on 16S rRNA clone libraries revealed that the Gram-positive bacteria represented by probe HGC69A-hybridized Actinobacteria (8.5 +/- 1.4% of total 4', 6-diamidino-2-phenylindole (DAPI)-stained cells) and probe LGC354-hybridized Firmicutes (3.8 +/- 0.8%) were the major phylogenetic bacterial phyla, followed by Bacteroidetes (4.0 +/- 1.2%) and Chloroflexi (3.7 +/- 0.8%). The probe MX825-hybridized Methanosaeta (7.6 +/- 0.8%) was the most abundant archaeal group, followed by Methanomicrobiales (2.8 +/- 0.6%) and Methanobacteriaceae (2.7 +/- 0.4%). The functional community structures (diversity and relative abundance) of major trophic groups were quantitatively analyzed by MAR-FISH. The results revealed that glucose-degrading microbial community had higher abundance (ca. 10.6 +/- 4.9% of total DAPI-stained cells) and diversity (at least seven phylogenetic groups) as compared with fatty acid-utilizing microbial communities, which were more specialized to a few phylogenetic groups. Despite the dominance of Betaproteobacteria, members of Chloroflexi, Smithella, Syntrophomonas and Methanosaeta groups dominated the [C-14]glucose-, [C-14]propionate-, [C-14]butyrate- and [C-14]acetate-utilizing microorganism community, and accounted for 27.7 +/- 4.3%, 29.6 +/- 7.0%, 34.5 +/- 7.6% and 18.2 +/- 9.5%, respectively. In spite of low abundance (ca. 1%), the hitherto unknown metabolic functions of Spirochaeta and candidate phylum of TM7 as well as Synergistes were found to be glucose and acetate utilization, respectively. (c) 2007 Elsevier Ltd. All rights reserved.