Distribution of Organic Cation Transporter 3, a Corticosterone-Sensitive Monoamine Transporter, in the Rat Brain
Distribution of Organic Cation Transporter 3, a Corticosterone-Sensitive Monoamine Transporter, in the Rat Brain
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DOI：
10.1002/cne.21921
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发表时间：
2009-02-01
影响因子：
2.5
通讯作者：
Lowry, Christoprer A.
中科院分区：
文献类型：
--
作者：
Gasser, Paul J.;Orchinik, Miles;Lowry, Christoprer A.
有机阳离子转运蛋白3（OCT 3）是一种高容量、亲和性转运蛋白，介导多巴胺、去甲肾上腺素、肾上腺素、5-羟色胺和组胺的双向、独立转运。皮质酮直接抑制转运，皮质酮是转运蛋白在调节应激和糖皮质激素对神经传递的某些作用中的潜在作用。为了阐明OCT 3清除脑中细胞外单胺的重要性，我们采用免疫组织化学技术描述了成年雄性大鼠脑吻尾侧范围内OCT 3样免疫反应（OCT 3-ir）细胞的作用。细胞体广泛分布于下丘脑的上级下丘、Calleia岛、下托、侧转、下丘脑外侧核和背内侧核、主嗅球和副嗅球的颗粒细胞层、小脑和压后颗粒皮质。在脑室周围器官中也观察到OCT 3-ir细胞和/或纤维，并且在所有脑室的衬里中观察到OCT 3-ir室管膜细胞。OCT 3-IR细胞体的广泛分布，包括接受密集的单胺能投射的区域，表明这种转运蛋白在调节大鼠脑中细胞外单胺浓度中的重要作用，并且与皮质酮诱导的OCT 3介导的转运抑制可能有助于急性应激或皮质酮对单胺能神经传递的影响的假设一致。J. Comp.神经元512：529-555，2009. (c)2008 Wiley-Liss，Inc.
Organic cation transporter 3 (OCT3) is a high-capacity, affinity transporter that mediates bidirectional, independent transport of dopamine, norepinephrine, nephrine, serotonin, and histamine. transport is directly inhibited by corticosterone, a potential role for the transporter in mediating some of effects of stress and glucocorticoids on neurotransmission. To elucidate the importance of OCT3 clearance of extracellular monoamines in the brain, we immunohistochemical techniques to describe the tion of OCT3-like-immunoreactive (OCT3-ir) cells out the rostrocaudal extent of adult male rat brains. cell bodies were widely distributed throughout the with the highest densities observed in the superior inferior colliculi, islands of Calleia, subiculum, lateral turn, lateral and dorsomedial hypothalamic nuclei, and granule cell layers of the main and accessory olfactory bulbs, the cerebellum, and the retrosplenial granular cortex. OCT3-ir cells and/or fibers were also observed in circumventricular organs, and OCT3-ir ependymal cells were observed in the linings of all cerebral ventricles. The widespread distribution of OCT3-ir cell bodies, including regions receiving dense monoaminergic projections, suggests an important role for this transporter in regulating extracellular concentrations of monoamines in the rat brain and is consistent with the hypothesis that corticosterone-induced inhibition of OCT3-mediated transport may contribute to effects of acute stress or corticosterone on monoaminergic neurotransmission. J. Comp. Neurol. 512:529-555, 2009. (c) 2008 Wiley-Liss, Inc.