The Ino80 complex mediates epigenetic centromere propagation via active removal of histone H3.
The Ino80 complex mediates epigenetic centromere propagation via active removal of histone H3.
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DOI：
10.1038/s41467-017-00704-3
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发表时间：
2017-09-13
影响因子：
16.6
通讯作者：
Lee D
中科院分区：
文献类型：
--
作者：
Choi ES;Cheon Y;Kang K;Lee D
着丝粒是染色体上的一个位点，在此位点上，动粒被组装以指导染色体分离。已知组蛋白H3变体，着丝粒蛋白A（CENP-A），在表观遗传学上标记活性着丝粒，但是CENP-A在着丝粒处繁殖，取代组蛋白H3的机制仍然知之甚少。使用裂殖酵母，在这里，我们表明，Ino 80腺苷三磷酸（ATP）依赖的染色质重塑复合物，去除组蛋白H3含有核小体相关的染色质，促进CENP-ACnp 1染色质组装在着丝粒在一个冗余的方式与另一个染色质重塑因子Chd 1Hrp 1。CENP-ACnp 1染色质主动募集Ino 80复合物至着丝粒，以引起含组蛋白H3的核小体的驱逐。将Ino 80亚基人工靶向至置于天然着丝粒中的非着丝粒DNA序列增强了CENP-ACnp 1染色质向非着丝粒DNA中的扩散。基于这些结果，我们提出CENP-ACnp 1染色质采用Ino 80复合物介导CENP-ACnp 1替换组蛋白H3，从而增强自身。组蛋白变体CENP-A标记活跃的着丝粒，并通过一种知之甚少的机制在着丝粒处取代H3。在这里，作者提供的证据表明，染色质重塑Ino 80促进CENP-A染色质组装在着丝粒分裂酵母。
The centromere is the chromosomal locus at which the kinetochore is assembled to direct chromosome segregation. The histone H3 variant, centromere protein A (CENP-A), is known to epigenetically mark active centromeres, but the mechanism by which CENP-A propagates at the centromere, replacing histone H3, remains poorly understood. Using fission yeast, here we show that the Ino80 adenosine triphosphate (ATP)-dependent chromatin-remodeling complex, which removes histone H3-containing nucleosomes from associated chromatin, promotes CENP-ACnp1 chromatin assembly at the centromere in a redundant manner with another chromatin-remodeling factor Chd1Hrp1. CENP-ACnp1 chromatin actively recruits the Ino80 complex to centromeres to elicit eviction of histone H3-containing nucleosomes. Artificial targeting of Ino80 subunits to a non-centromeric DNA sequence placed in a native centromere enhances the spreading of CENP-ACnp1 chromatin into the non-centromeric DNA. Based on these results, we propose that CENP-ACnp1 chromatin employs the Ino80 complex to mediate the replacement of histone H3 with CENP-ACnp1, and thereby reinforces itself. The histone variant CENP-A marks active centromeres and replaces H3 at centromeres through a poorly understood mechanism. Here, the authors provide evidence that the chromatin remodeller Ino80 promotes CENP-A chromatin assembly at the centromere in fission yeast.
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影响因子：
4.5
作者：
Buchanan L;Durand-Dubief M;Roguev A;Sakalar C;Wilhelm B;Strålfors A;Shevchenko A;Aasland R;Shevchenko A;Ekwall K;Francis Stewart A
通讯作者：
Francis Stewart A
DOI：
10.1126/science.1259308
发表时间：
2015-05-08
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Falk SJ;Guo LY;Sekulic N;Smoak EM;Mani T;Logsdon GA;Gupta K;Jansen LE;Van Duyne GD;Vinogradov SA;Lampson MA;Black BE
通讯作者：
Black BE
影响因子：
4.5
作者：
Catania S;Pidoux AL;Allshire RC
通讯作者：
Allshire RC
影响因子：
64.5
作者：
Black BE;Cleveland DW
通讯作者：
Cleveland DW
影响因子：
7.8
作者：
Jansen, Lars E. T.;Black, Ben E.;Foltz, Daniel R.;Cleveland, Don W.
通讯作者：
Cleveland, Don W.