Identification of genes differentially over-expressed in lung squamous cell carcinoma using combination of cDNA subtraction and microarray analysis
Identification of genes differentially over-expressed in lung squamous cell carcinoma using combination of cDNA subtraction and microarray analysis
复制标题
DOI：
10.1038/sj.onc.1203457
复制
发表时间：
2000-03-16
期刊：
影响因子：
8
通讯作者：
Reed, SG
中科院分区：
文献类型：
--
作者：
Wang, T;Hopkins, D;Reed, SG
为了开发针对人类癌症的有效疫苗产品，我们有兴趣鉴定肿瘤细胞中过表达的基因。通过cDNA文库消减和基因芯片技术相结合，我们发现了17个在肺鳞癌中优先表达的基因，其中包括4个新基因。迄今为止，这些基因的表达谱已通过北方和/或实时分析证实，并且还发现几个基因在头颈部鳞状肿瘤中表达。因此，这些组合方法代表了用于鉴定肿瘤特异性基因的高通量方法。此外，对这些基因的表征报告将允许它们被开发用于其诊断、预后和治疗潜力，包括免疫疗法和基于抗体的抗癌疗法。
In order to develop effective vaccine products against human cancer, we are interested in identifying genes over-expressed in tumor cells. Through a combination of cDNA library subtraction and microarray technology, we identified seventeen genes preferentially expressed in lung squamous cell carcinoma, including four novel genes. To date, expression profiles of these genes were confirmed by Northern and/or real-time analysis, and several genes were also found to be expressed in head and neck squamous tumors. Thus, these combined methods represent a high throughput approach for identifying tumor specific genes. Furthermore, the report of characterization on these genes will allow them to be exploited for their diagnostic, prognostic, and therapeutic potentials including immunotherapy and antibody based anticancer therapy.