Photothermal stress triggered by near infrared-irradiated carbon nanotubes promotes bone deposition in rat calvarial defects
Photothermal stress triggered by near infrared-irradiated carbon nanotubes promotes bone deposition in rat calvarial defects
复制标题
DOI：
10.1177/0885328214556913
复制
发表时间：
2015-03
影响因子：
2.9
通讯作者：
Tsukasa Yanagi;H. Kajiya;M. Kawaguchi;H. Kido;T. Fukushima
中科院分区：
文献类型：
--
作者：
Tsukasa Yanagi;H. Kajiya;M. Kawaguchi;H. Kido;T. Fukushima
骨再生愈合过程通常会延长，感染风险很高，尤其是老年和患病患者。缩短愈合过程时间通常需要机械应力装置、化学线索或激光/热疗法。尽管这些方法已广泛用于缩短骨愈合时间，但热应力诱导骨再生的确切机制仍不清楚。在这项研究中，我们研究了最佳热疗对体内大鼠颅骨缺损和体外成骨的影响。使用一种新型光热装置进行光热应激刺激，该装置由含有碳纳米管的藻酸盐凝胶及其近红外光照射器组成。与非热应力对照相比，近红外诱导碳纳米管触发的光热应力（42℃，每天 15 分钟）促进了临界尺寸颅骨缺损的骨沉积。我们最近报道，我们的新型 DNA/鱼精蛋白复合物支架可诱导颅骨缺损的骨再生。在这项研究中，光热应激上调了 DNA/鱼精蛋白移植颅骨缺损中的骨沉积。此外，光热应激以时间依赖性方式显着诱导成骨相关基因的表达，包括碱性磷酸酶、osterix和骨钙素。这在 DNA/鱼精蛋白细胞以及人 MG63 前成骨细胞中观察到，DNA/鱼精蛋白细胞是从移植到 DNA/鱼精蛋白支架的再生组织中扩增出来的。总之，这种基于碳纳米管的新型光热应激方法上调前成骨细胞中成骨相关基因的表达，从而促进矿物质沉积，从而增强骨修复。
The bone regenerative healing process is often prolonged, with a high risk of infection particularly in elderly and diseased patients. A reduction in healing process time usually requires mechanical stress devices, chemical cues, or laser/thermal therapies. Although these approaches have been used extensively for the reduction of bone healing time, the exact mechanisms involved in thermal stress-induced bone regeneration remain unclear. In this study, we investigated the effect of optimal hyperthermia on rat calvarial defects in vivo and on osteogenesis in vitro. Photothermal stress stimulation was carried out using a new photothermal device, composed of an alginate gel including in carbon nanotubes and their irradiator with near-infrared light. Photothermal stress (15 min at 42℃, every day), trigged by near-infrared-induced carbon nanotube, promoted bone deposition in critical-sized calvarial defects compared with nonthermal stress controls. We recently reported that our novel DNA/protamine complex scaffold induces bone regeneration in calvarial defects. In this study, photothermal stress upregulated bone deposition in DNA/protamine-engrafted calvarial defects. Furthermore, photothermal stress significantly induced expression of osteogenic related genes in a time-dependent manner, including alkaline phosphatase, osterix, and osteocalcin. This was observed in DNA/protamine cells, which were expanded from regenerated tissue engrafted into the DNA/protamine scaffold, as well as in human MG63 preosteoblasts. In summary, this novel carbon nanotube-based photothermal stress approach upregulated expression of osteogenic-related genes in preosteoblasts, resulting in promotion of mineral deposition for enhanced bone repair.