REEVALUATION OF A METHOD CALCULATING CLEAVAGE RATES AT DIFFERENT SITES OF DNA FROM PARTIAL DIGESTION OF END-LABELED MOLECULE
REEVALUATION OF A METHOD CALCULATING CLEAVAGE RATES AT DIFFERENT SITES OF DNA FROM PARTIAL DIGESTION OF END-LABELED MOLECULE
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DOI：
10.1016/s0006-291x(86)80564-2
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发表时间：
1986-07-31
影响因子：
3.1
通讯作者：
KARLOVSKY, P
中科院分区：
文献类型：
--
作者：
KARLOVSKY, P
核酸酶在DNA分子的不同部分以不同的速率切割相同的靶标。切割速率可以从DNA部分片段中出现的特定片段的量计算出来，但这些片段非常复杂，难以分离。为了简化分析，吕特建议使用末端标记的DNA分子（9）。在目前的通信中表明，Lutter的方法的基本方程是错误的。导出了正确的公式，并推荐用于重新计算以前的结果。
Nucleases cleave the same targets at different rates in different parts of DNA molecules. The cleavage rates can be calculated from the amounts of particular fragments occurring in the DNA partial digests, but these are very complex and difficult to separate. To simplify the analysis, the use of end-labelled DNA molecules was suggested by Lutter (9). It is shown in the present communication that the fundamental equation of Lutter''s approach is erroneous. A correct equation is derived and recommended to be used for re-calculation of the results obtained previously.