Brachyury-downstream gene sets in a chordate, Ciona intestinalis:: integrating notochord specification, morphogenesis and chordate evolution
Brachyury-downstream gene sets in a chordate, Ciona intestinalis:: integrating notochord specification, morphogenesis and chordate evolution
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DOI：
10.1111/j.1525-142x.2007.00212.x
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发表时间：
2008-01-01
影响因子：
2.9
通讯作者：
Gojobori, Takashi
中科院分区：
文献类型：
--
作者：
Hotta, Kohji;Takahashi, Hiroki;Gojobori, Takashi
在脊椎动物中，T盒转录因子基因brachyury似乎在中轴线中胚层细胞分化为脊索和原肠形成细胞运动中具有双重作用，受非规范Wnt/平面细胞极性(Wnt/PCP)信号调节。为了了解短链蛋白下游基因在脊索动物胚胎中的功能，通过对差异表达的一系列研究，包括消减杂交、斑点杂交、EST序列和整体原位杂交在胚胎阶段的表达模式，我们建立了一个名为“CINOBI：Ciona notochord and brachyury-下游基因索引”的知识库，以建立全面的短链蛋白下游基因集目录。结合基因组和大规模的cDNA数据，我们能够表征450个非冗余的短链下游基因：24个基因被新注释为脊索表达的基因。一些基因是信号通路的组成部分，如Wnt/PCP、核因子kappaB和转化生长因子-β信号转导。我们认为短毛动物与这些调节每个成分表达的途径有关，这种调节机制可能在脊索动物中是保守的。
In vertebrates, Brachyury, a T-box transcription factor gene, seems to have a dual role in the differentiation of axial midline mesoderm cells into notochord and gastrulation cell movements regulated by non-canonical Wnt/planar cell polarity (Wnt/PCP) signaling. To understand the function of Brachyury-downstream genes in chordate embryos, from a series of our survey on differential expression, including subtractive hybridization, dot-blot assays, EST sequences and the expression patterns in whole-mount in situ hybridization at embryonic stages, we developed a knowledge database called "CINOBI: Ciona Notochord and Brachyury-downstream gene Index" to create comprehensive catalogues of Brachyury-downstream gene sets in Ciona intestinalis. Combining genome and large-scale cDNA data, we were able to characterize 450 non-redundant Brachyury-downstream genes: Twenty-four genes were newly annotated as notochord-expressed genes. Several genes are components of signaling pathways such as Wnt/PCP, Nf kappa B and TGF-beta signaling. We propose that Brachyury is linked to these pathways regulating the expression of each component, and such a regulatory mechanism might be conserved among chordates.