Influence of Phosphatidylethanolamine Concentration and Composition on the Detection of Antiphosphatidylethanolamine Antibodies by ELISA.
Influence of Phosphatidylethanolamine Concentration and Composition on the Detection of Antiphosphatidylethanolamine Antibodies by ELISA.
复制标题
磷脂酰乙醇胺浓度和组成对 ELISA 检测抗磷脂酰乙醇胺抗体的影响。
DOI：
10.1002/jcla.21923
复制
发表时间：
2016
影响因子：
2.7
通讯作者：
Zhao,Ming
中科院分区：
文献类型：
--
作者：
Ke,Ke;Strango,ZachariahI;Harper,PaulE;Zhao,Ming
BackgroundAccumulating evidence supports a positive correlation between the presence of antiphosphatidylethanolamine (aPE) autoantibodies and clinical symptoms of antiphospholipid syndrome (APS). However, there is a lack of standardized ELISA‐based method for detecting aPE. The current study was conducted to investigate the dependence of aPE ELISA on lipid concentration and composition of PE antigens.MethodsA range of ELISA conditions were examined by varying the concentrations of egg PE and by substituting egg PE with combinations of synthetic DOPE and DSPE. The physical properties of the synthetic PE species were also characterized.ResultsOur data indicated that there are different optimal PE concentrations for conducting ELISA assays for cofactor‐dependent and cofactor‐independent aPEs. In addition, using a two‐component synthetic lipid system, we demonstrated aPE ELISA readouts can be modulated to approach the performance level of egg PE, which is currently the most commonly used PE antigen.ConclusionThese data raised the possibility of ultimately replacing natural PE antigens with a blend of defined synthetic lipid species, thus overcoming a known variable factor in aPE detection. The outcome of this study will help pave the way to developing a standardized aPE test.
