New method for standardization of electropherograms obtained in capillary zone electrophoresis
New method for standardization of electropherograms obtained in capillary zone electrophoresis
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DOI：
10.1016/s0021-9673(00)00585-9
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发表时间：
2000-10-13
影响因子：
4.1
通讯作者：
Hirokawa, T
中科院分区：
文献类型：
--
作者：
Ikuta, N;Yamada, Y;Hirokawa, T
提出了一种新的毛细管区带电泳电泳图谱标准化方法，即用有效迁移率轴代替迁移时间轴。通过引入迁移率表达式的温度系数和延迟时间，成功地消除了由于焦耳加热引起的温度升高引起的迁移率增加和电势梯度的弛豫效应。对模型样品评价了通过所提出的转换方法评价的迁移率的精度。通过使用该转换方法，即使从最初通过使用不同硬件条件获得的电泳图也可以获得几乎相同的电泳图。(C)2000 Elsevier Science B.V.保留所有权利。
A new method for standardization of electropherograms obtained by capillary zone electrophoresis was proposed, where the migration time axis was replaced by the effective mobility axis. The mobility increase due to temperature increase by Joule heating and the relaxation effect of the potential gradient were eliminated successfully by introducing a temperature coefficient for mobility expression and a delay time, respectively. The precision of the mobility evaluated by the proposed conversion methods was evaluated for a model sample. By using the conversion method, almost the same electropherograms could be obtained even from the electropherograms originally obtained by using different hardware conditions. (C) 2000 Elsevier Science B.V. All rights reserved.