Microbial associations in gut systems of wood- and bark-inhabiting longhorned beetles [Coleoptera: Cerambycidae]
Microbial associations in gut systems of wood- and bark-inhabiting longhorned beetles [Coleoptera: Cerambycidae]
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DOI：
10.1016/j.syapm.2009.10.002
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发表时间：
2010-01-01
影响因子：
3.4
通讯作者：
Hoell, W.
中科院分区：
文献类型：
--
作者：
Gruenwald, S.;Pilhofer, M.;Hoell, W.
利用荧光原位杂交（FISH）技术和基于PCR的rDNA序列分析，对树皮和木材上的天牛幼虫（Rhagium inquisitor、栗实天牛Teacherocastaneum、Plagionotus vulatus和Leptura rubra）肠道菌群进行了研究。从肠道内容物中分离出12株新的子囊酵母菌株。全真菌和菌株特异性寡核苷酸探针鉴定了两种酵母菌株，分别为Rhagii和Candida shehatae，它们定植于粘附在R. inquisitor和L. rubra幼虫。含有这些生物体的碎片不断地从菌丝体释放到肠腔中。而T castaneum的mycetome共生体不能被识别，这个物种的所有幼虫窝藏在他们的肠道上皮细胞的细菌内吞生物体。该菌属于Sodalis分支，包括舌蝇Sodalis glossinidius的次级内共生体和美洲象Sitophilus granulosa的初级内共生体，其肠道外植物群由α-、β-和γ-变形菌门、放线菌门、厚壁菌门、疣微菌门和酸杆菌门组成。然而，调查幼虫之间的个人组成是高度可变的，据推测取决于个别主机的营养。(C)2009年Elsevier GmbH。All rights reserved.
Using fluorescence in situ hybridization (FISH) techniques and PCR-based rDNA sequencing, gut microflora in the larvae of bark- and wood-inhabiting cerambycid beetles (Rhagium inquisitor, Tetropium castaneum, Plagionotus arcuatus and Leptura rubra [Coleoptera: Cerambycidae]) was investigated. A total of 12 novel ascomycetous yeast strains were isolated from the gut content. Panfungal and strain-specific oligonucleotide probes identified two yeast strains as Candida rhagii and Candida shehatae, which were colonizing specialized organs (mycetomes) adhering to the gut of R. inquisitor and L. rubra larvae, respectively. Fragments containing these organisms were constantly being released from the mycetomes into the gut lumen. Whereas the mycetome symbiont of T castaneum could not be identified, all larvae of this species harbored an additional bacterial endocytobiont in their gut epithelium. This novel gammaproteobacterium belonged to the Sodalis clade of insect symbionts, which includes the secondary endosymbiont of tsetse flies (Sodalis glossinidius) and the Sitophilus oryzae primary endosymbiont (SOPE).Extracellular gut flora of the investigated cerambycid larvae was comprised of Alpha-, Beta-, and Gammaproteobactetia, Actinobacteria, Firmicutes, Verrucomicrobia and Acidobacteria. However, the individual composition among investigated larvae was highly variable and supposedly depended on individual host nutrition. (C) 2009 Elsevier GmbH. All rights reserved.