The hypocretins: Hypothalamus-specific peptides with neuroexcitatory activity
The hypocretins: Hypothalamus-specific peptides with neuroexcitatory activity
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DOI：
10.1073/pnas.95.1.322
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发表时间：
1998-01-06
影响因子：
11.1
通讯作者：
Sutcliffe, JG
中科院分区：
文献类型：
--
作者：
De Lecea, L;Kilduff, TS;Sutcliffe, JG
我们描述了一个下丘脑特异性的mRNA，编码前下丘脑泌素原，一对肽的推定前体，共享大量的氨基酸身份与肠激素促分泌素，下丘脑泌素（Hcrt）蛋白质产品被限制到背侧和外侧下丘脑区域的神经元细胞体。这些神经元的纤维分布在下丘脑后部，并投射到其他区域的多个目标，包括脑干和丘脑。Hcrt免疫反应性与突触处的大颗粒囊泡有关。其中一种Hcrt肽在应用于培养的突触耦联的下丘脑神经元时具有兴奋性，但不应用于海马神经元。这些观察结果表明，hypocretins在中枢神经系统内的功能作为神经递质。
We describe a hypothalamus-specific mRNA that encodes preprohypocretin, the putative precursor of a pair of peptides that share substantial amino acid identities with the gut hormone secretin, The hypocretin (Hcrt) protein products are restricted to neuronal cell bodies of the dorsal and lateral hypothalamic areas. The fibers of these neurons are widespread throughout the posterior hypothalamus and project to multiple targets in other areas, including brainstem and thalamus. Hcrt immunoreactivity is associated with large granular vesicles at synapses, One of the Hcrt peptides was excitatory when applied to cultured, synaptically coupled hypothalamic neurons, but not hippocampal neurons. These observations suggest that the hypocretins function within the CNS as neurotransmitters.