High expression of miR-21 in triple-negative breast cancers was correlated with a poor prognosis and promoted tumor cell in vitro proliferation
High expression of miR-21 in triple-negative breast cancers was correlated with a poor prognosis and promoted tumor cell in vitro proliferation
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DOI：
10.1007/s12032-014-0057-x
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发表时间：
2014-07-01
期刊：
影响因子：
3.4
通讯作者：
Du, Zhaohui
中科院分区：
文献类型：
--
作者：
Dong, Guizhi;Liang, Xiaoling;Du, Zhaohui
三阴性乳腺癌(TNBC)预后特别差，其分子特征的异质性日益被人们所认识。MicroRNA表达谱已被用于乳腺癌的分类和预后，许多显著上调的microRNAs，即miR-21，已被证实在非TNBCs中致癌。在本研究中，我们检测了TNBC标本中miR-21的水平，以及体外培养的TNBC细胞水平，进而确定miR-21在肿瘤细胞增殖、凋亡中的作用，进而确定PTEN可能是microRNA的靶点。结果表明，miR-21的表达普遍上调，且在TNBC中的表达呈异质性，与TNBC患者预后不良有关。进一步的结果表明，上调的miR-21在体外促进了肿瘤的增殖，抑制了细胞的凋亡。促凋亡的PTEN被证明是靶向的，并且下调了表达。因此，我们的发现强调了miR-21在TNBC中的致癌作用。
The triple-negative breast cancer (TNBC), with a particularly poor prognosis, is increasingly recognized as heterogeneous in molecular signatures. MicroRNA expression profiles have been used for the classification and prognostication of breast cancer, numerous significantly upregulated microRNAs, i.e. miR-21, have been verified oncogenic in non-TNBCs. In present study, we determined the miR-21 levels in TNBC specimens, and TNBC cell levels in vitro, and then identified the role of miR-21 on tumor cell proliferation, apoptosis, and then identified PTEN as the possible target of the microRNA. It was shown that miR-21 expression is upregulated generally, and heterogeneous in TNBC specimens, posing a correlation with poor prognosis for TNBC patients. Further results demonstrated that the upregulated miR-21 promoted the tumor proliferation and inhibited cell apoptosis in vitro. And pro-apoptotic PTEN had been shown being targeted and downregulated. Therefore, our finding emphasized the oncogenic role of miR-21 in TNBC.