Construction of a SNP-Based High-Density Genetic Map Using Genotyping by Sequencing (GBS) and QTL Analysis of Nut Traits in Chinese Chestnut (Castanea mollissima Blume).
Construction of a SNP-Based High-Density Genetic Map Using Genotyping by Sequencing (GBS) and QTL Analysis of Nut Traits in Chinese Chestnut (Castanea mollissima Blume).
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DOI：
10.3389/fpls.2018.00816
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发表时间：
2018
影响因子：
5.6
通讯作者：
Qin L
中科院分区：
文献类型：
--
作者：
Ji F;Wei W;Liu Y;Wang G;Zhang Q;Xing Y;Zhang S;Liu Z;Cao Q;Qin L
板栗是一种分布广泛、经济价值十分重要的坚果树种。板栗的大小和成熟期是板栗育种的主要目标。然而，与坚果性状相关的高密度连锁图谱和数量性状基因座（QTL）分析还不完善，阻碍了板栗育种的进展。本研究以我国板栗栽培品种燕山早丰和冠亭10号为材料，通过基因分型测序（GBS）技术构建了一张基于单核苷酸多态性（SNP）的高密度连锁图谱。该遗传连锁图谱由2,620个SNP标记组成，总长度为1078.06 cM，分布在12个连锁群中，平均标记距离为0.41 cM。根据连续两年的表型数据，共检测到17个控制坚果单果重（SNW）、坚果宽度（NW）、坚果厚度（NT）、坚果高度（NH）和成熟期（RP）的QTL。在17个QTL中，利用与NT和RP性状相关的qNT-I-1和qRP-B-1。在LG Ⅰ上还发现了一个位于23.97cM的多效QTL，该QTL可能同时控制SNW、NT和NW。本研究为板栗坚果大小和成熟期的高密度遗传定位和QTL定位提供了参考，为板栗分子标记辅助选择育种提供了参考。
Chinese chestnut is a wildly distributed nut species with importantly economic value. The nut size and ripening period are mainly desired breeding objectives in Chinese chestnut. However, high-density linkage maps and quantitative trait loci (QTL) analyses related to nut traits are less than satisfactory, which hinders progress in the breeding of Chinese chestnut. Here, a single nucleotide polymorphism (SNP)-based high-density linkage map was constructed through genotyping-by-sequencing (GBS) of an F1 cross between the two widely grown Chinese chestnut cultivars ‘Yanshanzaofeng’ and ‘Guanting No. 10’. The genetic linkage map consists of 2,620 SNP markers with a total length of 1078.06 cM in 12 linkage groups (LGs) and an average marker distance of 0.41 cM. 17 QTLs were identified for five nut traits, specifically single-nut weight (SNW), nut width (NW), nut thickness (NT), nut height (NH), and ripening period (RP), based on phenotypic data from two successive years. Of the 17 QTLs, two major QTLs, i.e., qNT-I-1 and qRP-B-1 related to the NT and RP traits, respectively, were exploited. Moreover, the data revealed one pleiotropic QTL at 23.97 cM on LG I, which might simultaneously control SNW, NT, and NW. This study provides useful benchmark information concerning high-density genetic mapping and QTLs identification related to nut size and ripening period, and will accelerate genetic improvements for nuts in the marker-assisted selection (MAS) breeding of Chinese chestnut.
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