Maternal behavior regulates long‐term hippocampal expression of BAX and apoptosis in the offspring
Maternal behavior regulates long‐term hippocampal expression of BAX and apoptosis in the offspring
复制标题
DOI：
10.1046/j.1471-4159.2002.01054.x
复制
发表时间：
2002-08
影响因子：
4.7
通讯作者：
I. Weaver;R. Grant;M. Meaney
中科院分区：
文献类型：
--
作者：
I. Weaver;R. Grant;M. Meaney
母亲护理中自然发生的变化会影响大鼠海马的发育。在本研究中，我们发现出生第一周母体舔/梳理行为（LG）的变化与 90 日龄雄性后代海马 BAX（第 1 组肿瘤坏死因子家族介导的细胞死亡效应子）表达的改变有关。低水平 LG 母亲的成年后代中蛋白质印迹的 BAX 样免疫反应性显着增加。产妇护理对 B 细胞淋巴瘤-2 (BCL-2)（II 组线粒体介导的细胞死亡抑制因子）或 BAD（III 组内质网介导的细胞死亡效应因子）的水平没有影响。细胞凋亡中最引人注目的生化事件是DNA断裂。末端脱氧核苷酸转移酶（Tdt）介导的dUTP生物素缺口末端标记（TUNEL）和4',6'-二脒基-2-苯基吲哚盐酸盐（DAPI）染色显示，海马齿状回和CA1区的TUNEL阳性细胞数量显着增加。 低层LG妈妈。总之，我们认为低水平 LG 母亲的后代的海马神经元可能更容易因细胞凋亡而丢失。
Naturally occurring variations in maternal care influence hippocampal development in the rat. In the present study we found that variations in maternal licking/grooming (LG) during the first week of life are associated with altered hippocampal expression of BAX (group‐1 tumor necrosis factor family mediated cell death effector) in 90‐day‐old male offspring. BAX‐like immunoreactivity on western blots is significantly increased in the adult offspring of low‐level LG mothers. There is no effect of maternal care on levels of either B‐cell lymphoma‐2 (BCL‐2) (group‐II mitochondria mediated cell death suppressor) or BAD (group‐III endoplasmic reticulum mediated cell death effector). The most striking biochemical event in apoptosis is DNA fragmentation. Terminal deoxynucleotidyl transerferase (Tdt)‐mediated dUTP‐biotin nick‐end labeling (TUNEL) and 4′,6′‐diamidino‐2‐phenylindole hydrochloride (DAPI) staining showed that the number of TUNEL‐positive cells in both the dentate gyrus and CA1 region of the hippocampus is significantly increased in the adult offspring of low‐level LG mothers. In conclusion, we propose that hippocampal neurons in the offspring of low‐level LG mothers may be more vulnerable to loss through apoptosis.