Harnessing mechanobiology of human pluripotent stem cells for regenerative medicine.
Harnessing mechanobiology of human pluripotent stem cells for regenerative medicine.
复制标题
利用人类多能干细胞的机械生物学进行再生医学。
DOI：
10.1021/cn5001155
复制
发表时间：
2014
影响因子：
5
通讯作者：
Fu,Jianping
中科院分区：
文献类型：
--
作者：
Sun,Yubing;Fu,Jianping
人类多能干细胞（hPSC）的最新进展为运动神经元（MN）相关神经退行性疾病的治疗打开了新的大门。然而，MN分化过程尚未完全理解。在这个观点中，我们强调设计具有精确控制的机械性能（例如刚性）的合成细胞培养表面的概念，以提高从hPSC分化MN的效率。新出现的证据有力地支持了机械生物学在控制干细胞命运中的有效作用。利用hPSC的固有机械敏感性与合成弹性体微柱阵列系统相结合，我们最近已经证明了从hPSC显著改善的MN分化。hPSC的机械转导机制是一个未探索的领域，可能涉及多个靶点和途径的协调和交叉调节，包括细胞表面受体、信号转导分子和核蛋白。我们设想，对MN退行性疾病的hPSC研究将受益于积累hPSC的机械生物学知识。
Recent advances in human pluripotent stem cells (hPSCs) open new doors for therapeutics of motor neuron (MN)-associated neurodegenerative diseases. However, the MN differentiation process is not yet completely understood. In this Viewpoint, we stress the concept of designing synthetic cell culture surfaces with precisely controlled mechanical properties (such as rigidity) to improve the efficiency of MN differentiation from hPSCs. Emerging evidence strongly supports the potent role of mechanobiology in controlling stem cell fate. Leveraging the intrinsic mechanosensitive properties of hPSCs in conjunction with a synthetic elastomeric micropost array system, we have recently demonstrated significantly improved MN differentiation from hPSCs. Mechanotransduction mechanisms of hPSCs are an unexplored territory and likely involve coordination and cross-regulations of multiple targets and pathways including cell surface receptors, signaling transduction molecules, and nuclear proteins. We envision that research in hPSCs for MN degenerative diseases will benefit from accumulating knowledge of mechanobiology of hPSCs.