Effects of zinc deficiency on the vallate papillae and taste buds in rats.
Effects of zinc deficiency on the vallate papillae and taste buds in rats.
复制标题
缺锌对大鼠腭乳头和味蕾的影响。
DOI：
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发表时间：
2001
期刊：
影响因子：
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通讯作者：
K. Lu
中科院分区：
文献类型：
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作者：
H. Chou;C. Chien;H. L. Huang;K. Lu
背景和目的
缺锌与多种临床并发症有关，包括味觉障碍、厌食、生长迟缓、皮肤变化和性腺功能减退。我们研究了缺锌引起的断奶和年轻成年雄性 Wistar 大鼠的味蕾形态变化。
方法
总共使用24只断奶大鼠和30只年轻成年大鼠。每个年龄组又进一步分为：对照组，喂食锌充足（50 ppm）饮食；缺锌（< 1 ppm）饮食组；以及锌充足配对喂养组，喂食与缺锌组相同量的食物。断奶大鼠喂养4周，幼年大鼠喂养6周。使用光学和透射电子显微镜分析了味蕾的形态测量和形态变化。
结果
光学显微镜显示各组之间的乳头大小和形态没有显着差异。在缺锌饮食和配对喂养组的断奶和年轻成年大鼠中，每个乳头的味蕾数量（每只动物）和味蕾的平均轮廓面积均显着小于相应的对照组（p < 0.05）。仅在喂食缺锌饮食的断奶大鼠的味蕾中观察到超微结构的变化，味蕾结构紊乱，味蕾细胞之间的细胞间隙变宽。饲喂缺锌饲料的断奶大鼠味蕾中I型味蕾细胞的比例从59%下降到39%，II型味蕾细胞的比例从25%下降到12%。 III型味蕾细胞超微结构未观察到明显变化。
结论
缺锌对断奶和青年大鼠以及充足饮食配对喂养的大鼠的主要影响是味蕾数量和大小的变化以及味蕾细胞的精细结构变化，特别是在断奶后的加速生长阶段。
BACKGROUND AND PURPOSE
Zinc deficiency is associated with multiple clinical complications, including taste disturbance, anorexia, growth retardation, skin changes, and hypogonadism. We investigated the zinc-deficiency-induced morphologic changes in the vallate taste buds of weanling and young adult male Wistar rats.
METHODS
A total of 24 weanling and 30 young adult rats were used. Each age group was further divided into a control group fed a zinc-adequate (50 ppm) diet, a zinc-deficient (< 1 ppm) diet group, and a zinc-adequate pair-fed group who were fed the same amount of food as that taken by the zinc-deficient group. Weanling rats were fed for 4 weeks and young adult rats were fed for 6 weeks. The morphometry and morphologic changes of vallate taste buds were analyzed using light and transmission electron microscopy.
RESULTS
Light microscopy revealed no significant difference in papilla size and morphology among the various groups. In both weanling and young adult rats in the zinc-deficient diet and pair-fed groups, the number of taste buds per papilla (per animal) and the average profile area of the taste bud were significantly smaller than those of the corresponding controls (p < 0.05). Ultrastructural changes were seen only in the taste buds of weanling rats fed the zinc-deficient diet, with derangement of the architecture of the taste bud and widening of the intercellular space between taste bud cells. The proportion of type I taste bud cells in the taste buds of weanling rats fed the zinc-deficient diet decreased from 59% to 39%, and that of type II taste bud cells decreased from 25% to 12%. No obvious changes in the ultrastructure of type III taste bud cells were observed.
CONCLUSIONS
The main effects of zinc deficiency in weanling and young adult rats and in adequate diet pair-fed rats were changes in the number and size of taste buds, and fine structure changes in the taste bud cells, especially during the accelerated growth stage after weaning.