Expression of adenomatous polyposis coli (APC) in tumorigenesis of human oral squamous cell carcinoma
Expression of adenomatous polyposis coli (APC) in tumorigenesis of human oral squamous cell carcinoma
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DOI：
10.1016/j.oraloncology.2004.04.011
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发表时间：
2004-10-01
期刊：
影响因子：
4.8
通讯作者：
Tachikawa, T
中科院分区：
文献类型：
--
作者：
Tsuchiya, R;Yamamoto, G;Tachikawa, T
已经观察到腺瘤性结肠息肉病（APC）肿瘤抑制基因的产物通过β-连环蛋白调节Wnt信号通路。在本研究中，我们试图阐明APC与口腔鳞状上皮癌变之间的关系。通过激光显微切割技术，对口腔鳞状细胞癌病例的正常鳞状上皮、上皮异型增生和鳞状细胞癌（SCC）的每一个靶组织进行了修复。在回收的细胞中，我们检测了APC和β-连环蛋白基因转录产物表达的变化，以及APC基因突变的存在。通过免疫组化方法分析APC和β-catenin蛋白的定位。我们发现APC基因转录产物的表达水平在口腔鳞状上皮癌变和口腔鳞状细胞癌分化过程中有明显的变化。此外，在癌变过程中APC蛋白的定位也发生了一些变化。然而，尚不清楚APC是否发生了变异。在口腔鳞状细胞癌分化过程中，β-连环蛋白基因转录产物的表达水平也发生了变化。结果提示，APC表达水平和细胞内定位的改变与口腔鳞状上皮的癌变及口腔鳞癌的恶性特征有关。然而，APC基因的突变可能不是口腔鳞状上皮癌变所必需的。(C)2004 Elsevier Ltd. All. rights reserved.
The product of the adenomatous polyposis coli (APC) tumor suppressor gene has been observed to regulate the Wnt signaling pathway through beta-catenin. In the present study, we attempted to clarify the relation between APC and the canceration of oral squamous epithelium. Each target tissue of normal squamous epithelium, epithelial dysplasia, and squamous cell carcinoma (SCC) was recovered the oral SCC case by laser microdissection. In recovered cells, we examined the change in expression of APC and beta-catenin gene transcription products, as well as the existence of mutations in APC gene. We analyzed the localization of each protein of APC and beta-catenin by immunohistochemical study. We found a clear change in the expression level of the gene transcription product of APC in canceration of oral squamous epithelium and the differentiation of oral SCC. In addition, there was some change in the localization of the APC protein in canceration. It was not clear, however, whether the APC was mutated. A change was also observed in the expression level of the beta-catenin gene transcription product during the differentiation of oral SCC. Our results suggest that the changes in the expression Level and the intracellular localization of APC are related to the canceration of oral squamous epithelium, and the malignant characterization of oral SCC. Mutations of the APC gene might not be indispensable, however, in canceration of oral squamous epithelium. (C) 2004 Elsevier Ltd. All. rights reserved.