COMBINED USE OF MICROINCINERATION + PRUSSIAN BLUE REACTION FOR MORE SENSITIVE HISTOCHEMICAL DEMONSTRATION OF IRON
COMBINED USE OF MICROINCINERATION + PRUSSIAN BLUE REACTION FOR MORE SENSITIVE HISTOCHEMICAL DEMONSTRATION OF IRON
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DOI：
10.1177/12.3.153
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发表时间：
1964-01-01
影响因子：
3.2
通讯作者：
ZIMMERMAN, LE
中科院分区：
文献类型：
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作者：
FENTON, RH;JOHNSON, FB;ZIMMERMAN, LE
将一块玻璃载玻片支撑一块未遮盖的、未染色的组织石蜡切片，放在石棉片上，使用Fisher或Meker燃烧器火焰的氧化锥小心地加热。当样品完全焚化后，用金属锅将其覆盖，并让其逐渐冷却。灰分可以在暗场照明下直接检测氧化铁。如果将焚烧后的样品首先浸入纤维素溶液中，风干，迅速通过95%的乙醇，然后放入蒸馏水中，可能会通过通常的普鲁士蓝反应来染铁。这种方法不仅揭示了组织中铁的存在，而且还表明了蛋白质结合的铁的存在，通常是不可染色的。当所有其他简单的组织化学方法都失败时，使用这种技术可以证明铁在组织中的存在（特别是少量存在时）。
A glass slide supporting an uncovered, unstained paraffin section of a tissue is placed on a sheet of asbestos and cautiously heated, using the oxidizing cone from the flame of a Fisher or Meker burner. When the specimen has been completely incinerated it is covered with a metal pan and allowed to cool gradually. The ash may be examined directly in darkfield illumination for iron oxide. If the incinerated specimen is first dipped into a celloidin solution, air dried, passed rapidly through 95% ethyl alcohol, and then placed in distilled water, it may be stained for iron by the usual Prussian blue reaction. This method not only reveals the presence of ferric iron in tissues but also indicates the presence of protein-bound, ordinarily nonstainable, iron. By use of this technique the presence of iron in tissues (particularly if present in small amounts) can be demonstrated when all other simple histochemical methods have failed.