Fabrication of resorbable microporous intravascular stents for gene therapy applications.
Fabrication of resorbable microporous intravascular stents for gene therapy applications.
复制标题
用于基因治疗应用的可吸收微孔血管内支架的制造。
DOI：
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发表时间：
1994
期刊：
影响因子：
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通讯作者：
R. Eberhart
中科院分区：
文献类型：
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作者：
G. Rajasubramanian;R. S. Meidell;C. Landau;M. Dollar;D. B. Holt;J. Willard;M. Prager;R. Eberhart
作者利用聚-L-乳酸（PLLA）/聚ε-己内酯（PCL）混合物生产了可吸收的微孔腔内支架。螺旋支架和管支架设计都是通过溶剂浇铸和浮选沉淀制造技术获得的。采用一系列 PLLA/PCL 混合比例和工艺变量来研究它们对机械性能、孔隙率和降解率的影响。与 PCL 含量较高的材料相比，PLLA 比例较高的聚合物共混物具有较高的弹性模量和极限拉伸强度，以及较低的伸长率、孔隙率和降解率。获得了具有适合血管壁展开和支撑的机械性能的支架。使用酸溶胀技术将聚环氧乙烷掺入这些装置中，打开孔结构并改善亲水特性，从而能够摄取重组腺病毒载体。 50:50 PLLA/PCL 混合支架浸有重组腺病毒（AdCMB beta Gal，编码大肠杆菌 β-半乳糖苷酶的核定位变体）。培养的 CV-1 细胞与浸有重组病毒的支架一起孵育，表达核定位的 β-半乳糖苷酶活性，证实吸收的病毒以感染形式从基质中释放，动力学表明这种设计的遗传增强血管内装置是可行的。
The authors have produced resorbable, microporous endoluminal stents from Poly-L-lactic acid (PLLA)/Poly epsilon-caprolactone (PCL) blends. Both helical and tube stent designs have been obtained by solvent casting and flotation-precipitation fabrication techniques. A range of PLLA/PCL blend ratios and process variables were employed to investigate their influence on mechanical properties, porosity, and degradation rate. Polymer blends with higher PLLA proportions exhibit higher elastic moduli and ultimate tensile strength, and lower elongation, porosity, and degradation rates than do materials with higher PCL content. Stents with suitable mechanical properties for deployment and support of the vessel wall were obtained. Poly(ethylene oxide) was incorporated into these devices using an acid swelling technique, opening the pore structure and improving the hydrophilic character, thereby enabling the uptake of recombinant adenoviral vectors. The 50:50 PLLA/PCL blended stents were impregnated with recombinant adenovirus (AdCMB beta Gal, encoding a nuclear localizing variant of Escherichia coli beta-galactosidase). Cultured CV-1 cells incubated with stents impregnated with the recombinant virus expressed nuclear localized beta-galactosidase activity, confirming that absorbed virus is released from the matrix in an infectious form, with kinetics suggesting that genetically enhanced endovascular devices of this design are feasible.