Assessing indocyanine green pharmacokinetics in mouse liver with a dynamic diffuse fluorescence tomography system
Assessing indocyanine green pharmacokinetics in mouse liver with a dynamic diffuse fluorescence tomography system
复制标题
使用动态漫射荧光断层扫描系统评估小鼠肝脏中吲哚菁绿的药代动力学
DOI：
10.1002/jbio.201800041
复制
发表时间：
2018-10-01
影响因子：
2.8
通讯作者：
Gao, Feng
中科院分区：
文献类型：
--
作者：
Zhang, Yanqi;Zhang, Limin;Gao, Feng
组织中的荧光药代动力学速率可以为评价器官功能提供额外的特异性和定量的生理和病理信息。该模式需要以动态方式工作的高灵敏度扩散荧光断层扫描（DFT），以最终从测量的药代动力学相关的时变边界强度中提取药代动力学速率，通常需要先验解剖结构的支持。本文基于动态DFT和X射线计算机断层扫描（XCT）技术研究吲哚菁绿色（ICG）在小鼠肝脏中的药代动力学：采用高灵敏度的动态DFT系统和4个并行的光电倍增管光子计数通道进行CT扫描，生成信息丰富的瞬时采样数据集;分析程序使用高斯-牛顿方案从重建的ICG浓度-时间曲线中提取药代动力学速率，以拟合二室模型。在体实验中，10只健康小鼠肝脏对ICG的摄取率和排泄率可用于定量评价肝功能。结果验证了图像测量系统和药代动力学分析方法的有效性。
Fluorescence pharmacokinetic rates in tissues can provide additional specific and quantitative physiological and pathological information for evaluating organ function. This modality requires a highly sensitive diffuse fluorescence tomography (DFT) working in dynamic way to finally extract the pharmacokinetic rates from the measured pharmacokinetics‐associated temporally varying boundary intensity, normally with the support of a priori anatomy. This paper is devoted to study pharmacokinetics of indocyanine green (ICG) in mouse liver based on synergistic dynamic‐DFT and X‐ray computer tomography (XCT): A highly sensitive dynamic DFT system of CT‐scanning mode working with parallel 4 photomultiplier‐tube photon‐counting channels generates informative and instantaneous sampling datasets; An XCT system provides priori information of the target localization for improvement of the reconstruction quality; An analysis procedure extracts the pharmacokinetic rates from the reconstructed ICG concentration‐time curves, using the Gauss‐Newton scheme for fitting to a 2‐compartment model. The uptake and excretion rates of ICG which were obtained in livers of 10 healthy mice in the in vivo experiments can be used to quantitatively evaluate liver function. The results can validate the effectiveness of both the imaging measurements system and pharmacokinetic analysis method.