Intraperitoneal injection of (−)-Epigallocatechin-3-gallate protects against light-induced photoreceptor degeneration in the mouse retina
Intraperitoneal injection of (−)-Epigallocatechin-3-gallate protects against light-induced photoreceptor degeneration in the mouse retina
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发表时间：
2017-03
期刊：
影响因子：
2.2
通讯作者：
Shounan Qi;Chenguang Wang;Delu Song;Ying Song;J. Dunaief
中科院分区：
文献类型：
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作者：
Shounan Qi;Chenguang Wang;Delu Song;Ying Song;J. Dunaief
（−）-表没食子儿茶素-3-没食子酸酯（EGCG）是绿色茶的主要儿茶素成分，据报道可延缓或预防某些形式的癌症、关节炎、心血管疾病和神经退行性疾病。在这项研究中，我们确定系统给予EGCG是否可以保护小鼠视网膜免受光损伤（LD）。方法BALB/cJ小鼠腹腔注射EGCG或生理盐水后，置于冷白色发光二极管（LED）灯（10，000 lux）下照射5 h。LD后7天，使用光学相干断层扫描（OCT）、组织学和视网膜电图（ERG）评估视网膜结构和功能。此外，在LD之前和LD之后24 h通过qPCR定量几种氧化应激基因的mRNA。结果OCT和显微镜下观察到了光感受器的形态学保护作用。与生理盐水对照组小鼠相比，EGCG组小鼠的所有三种波型的ERG振幅均显著较高，这表明EGCG保护视网膜功能。此外，qPCR结果表明，EGCG的管理可以增加抗氧化基因SOD 2的mRNA水平LD前和LD后24小时。结论腹腔注射EGCG可减轻强光对BALB/cJ小鼠视网膜的损伤，保护视网膜的结构和功能。
Purpose (−)-epigallocatechin-3-gallate (EGCG), a major catechin component of green tea, is reported to delay or prevent certain forms of cancer, arthritis, cardiovascular disease, and neurodegenerative disorders. In this study, we determined if systemically administered EGCG could protect the retina against light damage (LD) in mice. Methods BALB/cJ mice were treated with either EGCG or saline via intraperitoneal (IP) injection, and then placed under constant cool white light-emitting diode (LED) light (10,000 lux) for 5 h. Retinal structure and function were evaluated using optical coherence tomography (OCT), histology, and electroretinography (ERG) 7 days after LD. In addition, the mRNAs of several oxidative stress genes were quantified by qPCR before LD and 24 h after LD. Results OCT and photomicrographs of mouse retinas showed morphologic protection of photoreceptors. Mice in the EGCG group had significantly higher ERG amplitudes for all three wave types compared with mice in the saline control group, which indicated that EGCG protected retinal function. Furthermore, qPCR results showed that EGCG administration can increase the mRNA level of the antioxidant gene Sod2 before LD and 24 h after LD. Conclusions The IP injection of EGCG attenuated the detrimental effects of bright light on the retinas of BALB/cJ mice by protecting the structure and function of the retina.