Frequency and structure of p53 rearrangements in human osteosarcoma.
Frequency and structure of p53 rearrangements in human osteosarcoma.
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发表时间：
1990-12
期刊：
影响因子：
11.2
通讯作者：
C. Miller;A. Aslo;C. Tsay;D. Slamon;K. Ishizaki;J. Toguchida;T. Yamamuro;B. Lampkin;H. Koeffler-H.-Koe
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作者：
C. Miller;A. Aslo;C. Tsay;D. Slamon;K. Ishizaki;J. Toguchida;T. Yamamuro;B. Lampkin;H. Koeffler-H.-Koe
骨肉瘤是最常见的儿童骨癌（Tebbi, c.k, and Gaeta， J. pediatrics）。安。， 17:285-300, 1988)。使用Southern blot图谱，我们发现60例骨肉瘤中有11例（18%）的p53基因限制模式发生了改变，其中6例丢失了另一个p53等位基因。相比之下，在来自其他类型肉瘤的50个样本中未检测到p53基因的改变。50%的骨肉瘤细胞系（8个中的4个）也有一个p53等位基因的重排，第二个等位基因缺失，这些细胞没有检测到p53 mRNA。没有检测到p53基因改变的骨肉瘤细胞系含有丰富的p53转录物。综上所述，数据显示人类骨肉瘤可以有p53基因的重排；这些重排可能导致细胞生长失去正常的约束。
Osteosarcoma is the most frequent childhood bone cancer (Tebbi, C. K., and Gaeta, J. Pediatr. Ann., 17:285-300, 1988). Using Southern blot mapping, we found that 11 of 60 (18%) osteosarcomas had altered restriction patterns of the p53 gene and that six of these had loss of the other p53 allele. In contrast, no alteration of the p53 gene was detected in 50 samples from other types of sarcomas. Fifty % of osteosarcoma cell lines (4 of 8) also had gross rearrangements of one p53 allele with loss of the second allele, and these had no detectable p53 mRNA. Osteosarcoma cell lines with no detectable alteration of the p53 gene contained abundant p53 transcripts. Taken together, data show that human osteosarcomas can have rearrangements of the p53 gene; these rearrangements may cause loss of normal constraints on cellular growth.