SPONTANEOUS RECURRENT SEIZURES IN RATS - AMINO-ACID AND MONOAMINE DETERMINATION IN THE HIPPOCAMPUS
SPONTANEOUS RECURRENT SEIZURES IN RATS - AMINO-ACID AND MONOAMINE DETERMINATION IN THE HIPPOCAMPUS
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DOI：
10.1111/j.1528-1157.1994.tb02905.x
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发表时间：
1994-01-01
期刊：
影响因子：
5.6
通讯作者：
NAFFAHMAZZACORATTI, MG
中科院分区：
文献类型：
--
作者：
CAVALHEIRO, EA;FERNANDES, MJ;NAFFAHMAZZACORATTI, MG
毛果芸香碱全身给药所致的持续性惊厥所致的大鼠脑结构损伤是研究啮齿动物癫痫发作发生和传播的重要机制的有用模型。在给药后，观察到三个不同的阶段：(A)与反复边缘癫痫发作和癫痫持续状态相对应的1-2天的急性期；(B)以逐渐恢复到正常脑电和持续4-44天的行为为特征的无发作(沉默)期；(C)从给药后5-45天开始的自发性反复发作(SRS)期，持续整个动物的一生。Pilo(320-350 mg/kg，ip)给大鼠灌胃，用高效液相色谱法测定急性期、静止期和SRS期大鼠海马单胺和氨基酸的含量。去甲肾上腺素(NE)水平在各时期均呈下降趋势，而多巴胺(DA)水平则呈上升趋势。5-羟色胺(5-羟色胺，5-羟色胺)仅在急性期升高。单胺利用率测定显示，NE消耗增加，DA消耗减少。5-羟色胺利用率仅在急性期增加。氨基酸含量显示，在急性期，天冬氨酸(ASP)和谷氨酸(GLU)浓度随着γ-氨基丁酸(GABA)水平的升高而降低。静止期的特征是甘氨酸(GLY)和GABA水平下降，GLU浓度上升。SRS期显示出所有氨基酸浓度的增加。这些发现表明，在匹罗卡品引发的癫痫持续状态引起的脑损伤后，癫痫灶建立过程中的重要神经化学变化。
Rats subjected to structural brain damage induced by sustained convulsions triggered by systemic administration of pilocarpine (PILO) are a useful model for investigation of the mechanisms essential for seizure generation and spread in rodents. After PILO administration, three distinct phases are observed: (a) an acute period of 1-2 days' duration corresponding to a pattern of repetitive limbic seizures and status epilepticus; (b) a seizure-free (silent) period characterized by a progressive return to normal EEG and behavior of 4-44 days' duration; and (c) a period of spontaneous recurrent seizures (SRS) starting 5-45 days after PILO administration and lasting throughout the animal's life. PILO (320-350 mg/kg intraperitoneally, i.p.) was administered to rats, and the content of hippocampal monoamines and amino acids was measured in the acute, silent, and SRS periods by liquid chromatography. Norepinephrine (NE) level was decreased during all periods whereas dopamine (DA) content was increased. Serotonin (5-hydroxytryptamine, 5-HT) was increased only in the acute period. Utilization rate measurement of monoamines showed increased NE consumption and decreased DA consumption during all phases. 5-HT utilization rate was increased only in the acute period. Amino acid content showed a decrease in aspartate (ASP) and glutamate (GLU) concentrations associated with increased gamma-aminobutyric acid (GABA) level during the acute period. The silent phase was char acterized by a decrease in glycine (GLY) and GABA levels and an increase in GLU concentration. The SRS period showed an increase in all amino acid concentrations. These findings show important neurochemical changes in the course of establishment of an epileptic focus after brain damage induced by status epilepticus triggered by pilocarpine.