Rye chromosome variability in wheat-rye addition and substitution lines
Rye chromosome variability in wheat-rye addition and substitution lines
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DOI：
10.1023/a:1009299300018
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发表时间：
1999-01-01
影响因子：
2.6
通讯作者：
Vershinin, AV
中科院分区：
文献类型：
--
作者：
Alkhimova, AG;Heslop-Harrison, JS;Vershinin, AV
在黑麦染色体的多态性和稳定性研究中，利用两个非同源的高重复DNA家族pSc200和pSc250对三个黑麦品种和一组小麦-黑麦附加系和代换系进行了比较。黑麦品种Petkus、Imperial和Onohoiskaya的2R、4R和7R染色体上的pSc200原位杂交信号的存在和大小以及5R、6R和7R染色体上的pSc250信号的存在和大小都表现出多态。1R染色体在Onohoiskaya品种中是异形的。染色体多态分布的差异表明，这些高度重复的DNA家族的品种间变化是独立发生的，尽管它们在亚端粒异染色区并列甚至重叠。在Saratovskaya 29小麦/Onohoiskaya代换系中，由于长臂上的pSc250信号的扩增，仅2R染色体相对于其相应的亲本黑麦品种发生了改变，尽管这并未超过品种间的多态性。在中国春小麦/帝国附加系中，只有4R和6R两条帝国染色体保持不变。我们检测到一个或两个黑麦同源染色体的丢失，一条臂的丢失，以及伴随着pSc200信号丢失的亚端粒异染色质的缺失。结果表明，与中国春/帝国附加系相比，Saratovskaya 29/Onohoiskaya染色体置换系具有更高的染色体稳定性。
In a study of polymorphism and stability in rye chromosomes, three rye varieties and the sets of wheat-rye addition and substitution lines were compared using two non-homologous highly repetitive DNA families, pSc200 and pSc250. The rye varieties, Petkus, Imperial and Onohoiskaya, showed polymorphism for the presence and the size of the pSc200 in-situ hybridization signals on chromosome pairs, 2R, 4R and 7R, and the pSc250 signals on chromosomes, 5R, 6R and 7R. Chromosome 1R was heteromorphic within the Onohoiskaya variety. Differences in the distribution of chromosome polymorphisms imply that intervarietal changes to these highly repetitive DNA families occurred independently, despite their juxtaposition or even overlapping locations in subtelomeric heterochromatic regions. In the set of Saratovskaya 29 wheat/Onohoiskaya substitution lines, only chromosome 2R was altered relative to its counterpart in the parental rye variety due to amplification of the pSc250 signal on the long arm, although this did not exceed intervarietal polymorphism. In the set of Chinese Spring wheat/ Imperial addition lines, only two Imperial chromosomes, 4R and 6R, were unchanged. We detected the loss of one or both rye homologous chromosomes, the loss of one arm, and the deletion of subtelomeric heterochromatin accompanied by the loss of the pSc200 signal. The results show that Saratovskaya 29/Onohoiskaya chromosome substitution lines possess increased chromosome stability compared with Chinese Spring/Imperial addition lines.