Genetic variability in geographical populations of Aedes aegypti (Diptera, Culicidae) in Brazil elucidated by molecular markers
Genetic variability in geographical populations of Aedes aegypti (Diptera, Culicidae) in Brazil elucidated by molecular markers
复制标题
DOI：
10.1590/s1415-47572006000200030
复制
发表时间：
2006-01-01
影响因子：
2.1
通讯作者：
Ribolla, Paulo E.M.
中科院分区：
文献类型：
--
作者：
Paduan, Karina dos Santos;Araújo-Júnior, João P.;Ribolla, Paulo E.M.
热带蚊子埃及伊蚊是城市黄热病和登革热最重要的国内媒介。对这种病媒的遗传群体研究很重要，因为它们可能导致新的监测工具。对巴西6个联邦州4个人口区11个地点的埃及伊蚊种群进行了遗传结构分析。标记包括21个随机扩增多态性DNA （RAPD）位点。群体间检测到RAPD标记，聚类分析显示两大群体。结果表明，不同州和同一州的城市人群存在较高的遗传多态性（H-S = 0.224）和遗传分化（G(ST) = 0.430）。这些结果表明巴西境内埃及伊蚊种群存在显著差异。地理距离和RAPD标记的F-ST值的回归分析表明，遗传结构与地理定位存在相关性。
The tropical mosquito, Aedes aegypti is the most important domestic vector of urban yellow fever and dengue. Genetic population studies on this vector are important because they may lead to new tools for surveillance. An analysis of genetic structure was conducted among populations of A. aegypti from 11 localities in four demographic regions within six Brazilian federal states. Markers included 21 random amplified polymorphic DNA (RAPD) loci. RAPD markers were detected among populations and cluster analysis revealed two main groups. We found high genetic polymorphism (H-S = 0.224) and high levels of genetic differentiation between populations from different states (G(ST) = 0.430), as well as in populations from cities in the same state (G(ST) = 0.410). These results indicate significant differentiation in A. aegypti populations in Brazil. Regression analyses of geographic distances and pairwise F-ST values estimated from RAPD markers showed that there is a correlation between genetic structure and geographic localization.