Dopamine induces neurite retraction in retinal horizontal cells via diacylglycerol and protein kinase C.
Dopamine induces neurite retraction in retinal horizontal cells via diacylglycerol and protein kinase C.
复制标题
多巴胺通过二酰甘油和蛋白激酶 C 诱导视网膜水平细胞中的神经突回缩。
DOI：
10.1073/pnas.87.24.9693
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发表时间：
1990
影响因子：
11.1
通讯作者：
Dowling,JE
中科院分区：
文献类型：
--
作者：
Rodrigues,PdosS;Dowling,JE
多巴胺引起培养的牛头鲶鱼水平细胞的神经突明显收缩。氟哌啶醇和D1拮抗剂SCH 23390可阻断多巴胺的作用，但D2拮抗剂舒必利不能。D1激动剂SKF 38393可以模拟多巴胺诱导的形态学变化，而D2激动剂喹匹罗则不能。海钠酸盐也引起神经突收缩，但测试的其他神经活性物质包括谷氨酸、5-羟色胺、n -甲基- d -天冬氨酸、γ -氨基丁酸和甘氨酸只引起神经突长度的微小变化。环状AMP类似物不会诱导水平细胞的神经突收缩，这表明多巴胺的这种作用不是由环状AMP介导的。然而，蛋白激酶C激活剂（phorbol 12-豆蔻酸酯13-乙酸酯）和合成的二酰基甘油类似物（1-油基-2-乙酰基-森-甘油和二辛酸甘油）会引起明显的神经突收缩。它们的作用，以及多巴胺引起的变化，被一种有效的蛋白激酶拮抗剂staurosporine阻断。结果表明，多巴胺通过二酰基甘油激活蛋白激酶C引起神经突收缩。
Dopamine causes a significant retraction of neurites of bull-head catfish horizontal cells maintained in culture. The effects of dopamine are blocked by haloperidol and SCH 23390, a D1 antagonist, but not by sulpiride, a D2 antagonist. The dopamine-induced morphological changes were mimicked by SKF 38393, a D1 agonist, but not by quinpirole, a D2 agonist. Kainate also caused process retraction, but other neuroactive substances tested including glutamate, 5-hydroxytryptamine, N-methyl-D-aspartate, gamma-aminobutyric acid, and glycine caused only minor changes in neurite length. Cyclic AMP analogues do not induce neurite retraction in horizontal cells, indicating that this effect of dopamine is not mediated by cyclic AMP. However, a protein kinase C activator (phorbol 12-myristate 13-acetate) and synthetic diacylglycerol analogs (1-oleoyl-2-acetyl-sn-glycerol and dioctanoglycerol) caused marked neurite retraction. Their effects, as well as the dopamine-induced changes, were blocked by staurosporine, a potent protein kinase antagonist. The results suggest that dopamine causes neurite retraction by the activation of protein kinase C via diacylglycerol.