Biopolymer Monolith for protein separation
Biopolymer Monolith for protein separation
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用于蛋白质分离的生物聚合物整体料
DOI：
10.1039/c9fd00018f
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发表时间：
2019
影响因子：
3.4
通讯作者：
Y.
中科院分区：
文献类型：
--
作者：
Miura;Y.; Seto;H.; Hoshino;Y.
为了研究蛋白质的吸附和分离，以α-甘露糖、丙烯酰胺和交联剂的丙烯酰胺衍生物为原料，采用聚合诱导相分离的方法，通过自由基聚合法制备了多孔糖共聚物。多孔结构是由致孔剂乙醇诱导形成的。孔直径和比表面积受乙醇种类的影响。在间歇和连续流动系统中测量了蛋白质的吸附。糖核与刀豆蛋白A的糖识别蛋白具有特异性相互作用，与其他蛋白的非特异性相互作用可忽略不计。蛋白质在材料上的吸附量由糖的密度和糖粒的组成决定。获得了用于蛋白质分离的糖球的基本知识。
Porous glycopolymers, “glycomonoliths”, were prepared by radical polymerization based on polymerization-induced phase separation with an acrylamide derivative of α-mannose, acrylamide and cross-linker in order to investigate protein adsorption and separation. The porous structure was induced by a porogenic alcohol. The pore diameter and surface area were controlled by the type of alcohol. The protein adsorption was measured in both batch and continuous flow systems. The glycomonoliths showed specific interaction with the sugar recognition protein of concanavalin A, and non-specific interaction to other proteins was negligible. The amount of protein adsorption to the materials was determined by the sugar density and the composition of the glycomonoliths. Fundamental knowledge regarding the glycomonoliths for protein separation was obtained.