Development of the irradiation method for the first instar silkworm larvae using locally targeted heavy-ion microbeam
Development of the irradiation method for the first instar silkworm larvae using locally targeted heavy-ion microbeam
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DOI：
10.1269/jrr.06066
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发表时间：
2007-05-01
影响因子：
2
通讯作者：
Kiguchi, Kenji
中科院分区：
文献类型：
--
作者：
Fukamoto, Kana;Shirai, Koji;Kiguchi, Kenji
为了进行家蚕的放射显微外科研究，我们已经开发了针对卵和三至五龄幼虫的特定照射系统。在这项研究中，一个修改后的应用程序组成的一龄蚕幼虫进一步开发使用重离子微束。该系统包括专门设计用于在照射过程中固定一龄家蚕幼虫的带孔铝板，并且使用Mylar膜来调节沉积的能量以规划一定深度的辐射剂量。使用该系统，研究了对结突变体中表皮细胞异常增殖的抑制。用220 MeV碳（C-12）离子的180 μ m直径的微束对第一龄阶段的节形成区域进行靶照射后，饲养幼虫以评估照射效果。结果表明，120、250、400和600戈伊照射对5.9%、56.4%、66.7%和73.6%的幼虫照射节的形成有特异性抑制作用，而对未照射节的其他节的形成无抑制作用。虽然有些幼虫没有生存不希望的非靶向曝光，我们目前的研究结果表明，这种方法将是有用的，以调查在一个较长的发育期的辐射效应。此外，我们的系统也可以应用于其他物种的目标组织，或器官在发育和变态的昆虫和动物。
To carry out the radio-microsurgery study using silkworm, Bombyx mori, we have already developed the specific irradiation systems for eggs and third to fifth instar larvae. In this study, a modified application consisting of the first instar silkworm larvae was further developed using heavy-ion microbeams. This system includes aluminum plates with holes specially designed to fix the first instar silkworm larvae during irradiation, and Mylar films were used to adjust energy deposited for planning radiation doses at certain depth. Using this system, the suppression of abnormal proliferation of epidermal cells in the knob mutant was examined. Following target irradiation of the knob-forming region at the first instar stage with 180- tm-diameter microbeam of 220 MeV carbon (C-12) ions, larvae were reared to evaluate the effects of irradiation. The results indicated that the knob formation at the irradiated segment was specially suppressed in 5.9, 56.4, 66.7 and 73.6% of larvae irradiated with 120, 250, 400 and 600 Gy, respectively, but the other knob formations at the non-irradiated segments were not suppressed in either irradiation. Although some larva did not survive undesired non-targeted exposure, our present results indicate that this method would be useful to investigate the irradiation effect on a long developmental period of time. Moreover, our system could also be applied to other species by targeting tissues, or organs during development and metamorphosis in insect and animals.