Disruption of signaling by Yersinia effector YopJ, a ubiquitin-like protein protease
Disruption of signaling by Yersinia effector YopJ, a ubiquitin-like protein protease
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DOI：
10.1126/science.290.5496.1594
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发表时间：
2000-11-24
期刊：
影响因子：
56.9
通讯作者：
Dixon, JE
中科院分区：
文献类型：
--
作者：
Orth, K;Xu, ZH;Dixon, JE
耶尔森氏菌毒力效应子YopJ的同源物在植物和动物细菌病原体以及植物共生体中都有发现。这些YopJ家族成员被证明是半胱氨酸蛋白酶。该蛋白酶的催化三联体是抑制动物细胞中的丝裂原活化蛋白激酶（MAPK）和核因子κ B（NF-κ B）信号传导以及诱导植物中的局部细胞死亡所必需的。YopJ的底物被证明是高度保守的泛素样分子，其被共价地添加到许多调节蛋白。YopJ家族成员通过破坏这种翻译后修饰对细胞发挥其致病作用。
Homologs of the Yersinia virulence effector YopJ are found in both plant and animal bacterial pathogens, as well as plant symbionts. These YopJ family members were shown to act as cysteine proteases. The catalytic triad of the protease was required for inhibition of the mitogen-activated protein kinase (MAPK) and nuclear factor kappaB (NF-kappaB) signaling in animal cells and for induction of Localized cell death in plants. The substrates for YopJ were shown to be highly conserved ubiquitin-like molecules, which are covalently added to numerous regulatory proteins. YopJ family members exert their pathogenic effect on cells by disrupting this posttranslational modification.