APPLICATION OF ULTRASONICATION OR HIGH-PRESSURE EXTRACTION OF FLAVONOIDS FROM LITCHI FRUIT PERICARP
APPLICATION OF ULTRASONICATION OR HIGH-PRESSURE EXTRACTION OF FLAVONOIDS FROM LITCHI FRUIT PERICARP
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DOI：
10.1111/j.1745-4530.2008.00247.x
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发表时间：
2009-12-01
影响因子：
3
通讯作者：
Jiang, Yueming
中科院分区：
文献类型：
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作者：
Prasad, K. Nagendra;Yang, Bao;Jiang, Yueming
荔枝(Litchi chinensis Sonn.)果皮中含有大量的类黄酮类物质，可作为天然抗氧化剂。一些新兴的新技术，如超声波提取(UE)和高压提取(HPE)，在黄酮类化合物的提取方面显示出巨大的潜力。对比考察了常规萃取、40 kHz超临界流体萃取和200或400兆帕高压萃取对大豆黄酮类成分提取效率的影响。提取30min后，测定提取物得率、总酚含量、清除1，1-二苯基-2-苦基肼自由基能力和清除超氧阴离子能力。UE、400 HPE和CE的粗提物得率分别为24%、30%和1.83%。而同一浓度下三种不同提取物的总酚含量和抗氧化活性均无显著差异(P>；0.05)。此外，还鉴定了表没食子儿茶素没食子酸酯和表儿茶素没食子酸酯两种黄酮类化合物为主要成分，而次要成分为儿茶素和原花青素B-2。在200和400 MPS、UE和CE条件下，HPE法测得总黄酮含量分别为0.65、0.75、0.29和0.07 mg/g干重。结果表明，HPE提取LFP中黄酮类成分的效率较高，且提取时间短，酚类化合物的提取量大。是一种原产于东南亚的亚热带水果。随着荔枝果实因其美味的味道和诱人的红皮逐渐被消费者接受，近几十年来荔枝产量稳步增长，南半球和北半球产区对欧洲和北美的出口都有所增加。荔枝果皮(LFP)约占整个新鲜水果重量的16%，含有大量的类黄酮。因此，LFP组织可能被认为是膳食中类黄酮的重要来源。本研究表明，HPE技术对荔枝组织中黄酮类化合物的提取具有较高的效率，有助于荔枝产业开发新的提取方法，更好地利用荔枝组织中的黄酮类化合物。
Litchi (Litchi chinensis Sonn.) fruit pericarp (LFP) contains a high amount of flavonoids, which could be used as natural antioxidants. Some emerging novel technologies, such as ultrasonic extraction (UE) and high-pressure extraction (HPE), have exhibited great potential for flavonoid extraction. Experiments were conducted to comparatively investigate the effects of conventional extraction (CE), 40-KHz UE and 200- or 400-MPa HPE on the extraction efficiency of flavonoids from LFP. After 30 min of extraction, the extract yield, total phenolic content, 1,1-diphenyl-2-picrylhydrazyl radical scavenging activity and superoxide anion scavenging ability were examined. The crude extract yields by the UE, 400 HPE and CE were 24, 30 and 1.83%, respectively. However, there were no significant differences (P > 0.05) in the total phenolic content and antioxidant activity tested among these three different extractions at the same concentration. In addition, two flavonoids, namely epicatechin and epicatechin gallate, were identified and quantified as the major compounds, while catechin and procyanidin B-2 were identified as the minor compounds. The total flavonoid content detected was 0.65, 0.75, 0.29 and 0.07 mg/g dry weight by HPE at 200 and 400 MPs, UE, and CE, respectively. Thus, the HPE technique showed a higher efficiency in extracting flavonoids from LFP, and the HPE could reduce the extraction time and increase the amount of the extracted phenolics.PRACTICAL APPLICATIONSLitchi (Litchi chinensis Sonn.) is a subtropical fruit that originated in Southeast Asia. As litchi fruit is gradually accepted by consumers for its delicious taste and attractive red skin, litchi production has steadily increased in recent decades, with increasing exports to Europe and North America from both the southern and northern hemisphere production areas. Litchi fruit pericarp (LFP) accounts for approximately 16% by weight of the whole fresh fruit and is comprised of a significant amount of flavonoids. Therefore, LFP tissues may be considered an important source of dietary flavonoids. This work showed that HPE technique has a high efficiency in extracting flavonoids from LFP tissues, which may help the litchi industry develop new extraction methods to better utilize the flavonoids from LFP tissues.