The characterisation of eukaryotic microbial communities on sandstone buildings in Belfast, UK, using TRFLP and 454 pyrosequencing
The characterisation of eukaryotic microbial communities on sandstone buildings in Belfast, UK, using TRFLP and 454 pyrosequencing
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DOI：
10.1016/j.ibiod.2013.03.010
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发表时间：
2013-08-01
影响因子：
4.8
通讯作者：
Whiteley, Andrew S.
中科院分区：
文献类型：
--
作者：
Cutler, Nick A.;Oliver, Anna E.;Whiteley, Andrew S.
真核微生物，特别是微生物藻类和真菌，可以对建筑石材的生物降解产生重大影响，特别是当它们形成绿色生物膜时。然而，人们对生活在石头表面的真核生物群落的组成和结构知之甚少。本研究的双重目的是：a）描述生活在英国贝尔法斯特遗产建筑上的藻类和真菌群落的特征；b）研究真核群落组成与各种基质特征之间的关系。我们使用分子技术（TRFLP 和 454 焦磷酸测序）来表征群落。我们发现藻类群落的分类丰富度出人意料地高，但由于类群分布不公平，藻类和真菌组合的总体多样性水平较低。我们的研究结果表明，砂岩结构上存在一小部分世界性藻类物种和相对均匀的藻类群落。相比之下，真菌群落更加丰富，空间异质性也更强。 20 世纪 80 年代对其中一处建筑进行的强力化学清洁很可能对微生物群落结构产生了持续的影响。此外，虽然底物特征似乎影响真核微生物群落的丰度/生物量，但它们不影响多样性。 (c) 2013 Elsevier Ltd. 保留所有权利。
Eukaryotic microorganisms, notably microbial algae and fungi, can have a major impact on the biodeterioration of building stone, particularly when they form green biofilms. However, comparatively little is known about the composition and structure of eukaryotic communities living on the surface of stone. The twin aims of this study were to a) characterise algal and fungal:communities living on heritage structures in Belfast, UK and b) to investigate the relationship between eukaryotic community composition and a variety of substrate characteristics. We used molecular techniques (TRFLP and 454 pyrosequencing) to characterise the communities. We found unexpectedly high levels of taxonomic richness in algal communities, but low overall levels of diversity in both the algal and the fungal assemblages resulting from inequitable distributions of taxa. Our findings suggest the existence of a small pool of cosmopolitan algal species and relatively homogeneous algal communities on sandstone structures. In contrast, fungal communities were much richer and more spatially heterogeneous. It is likely that the aggressive chemical cleaning of one of the structures in the 1980s has had an ongoing impact on microbial community structure. Furthermore, whilst substrate characteristics seem to impact on the abundance/biomass of eukaryotic microbial communities, they do not influence diversity. (c) 2013 Elsevier Ltd. All rights reserved.