Hemoglobin adducts of acrylamide and acrylonitrile in laboratory workers, smokers and nonsmokers
Hemoglobin adducts of acrylamide and acrylonitrile in laboratory workers, smokers and nonsmokers
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DOI：
10.1021/tx960113p
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发表时间：
1997-01-01
影响因子：
4.1
通讯作者：
Bergmark, E
中科院分区：
文献类型：
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作者：
Bergmark, E
丙烯酰胺是一种广泛用于研究实验室制备电泳用聚丙烯酰胺凝胶（PAGE）的化学品。从从事PAGE工作的实验室人员、吸烟者和非吸烟者中采集血样。血红蛋白加合物的丙烯酰胺，丙烯腈和环氧乙烷的测定使用改进的埃德曼降解程序。在所有人中检测到丙烯酰胺加合物。PAGE工人（平均54 pmol/g）有一个显着增加的加合物水平相比，非吸烟对照组（平均31 pmol/g）。吸烟者的丙烯酰胺加合物（平均116 pmol/g）与每天吸烟的数量相关。这证实了烟草烟雾中存在丙烯酰胺，并表明它是丙烯酰胺暴露的重要来源。PAGE工作者中丙烯酰胺加合物水平的增加对应于每天从约3支香烟中摄取丙烯酰胺。从本研究中无法得出工作程序中哪个步骤对暴露最关键的结论。PAGE工人可能没有神经毒性损伤周围神经系统的风险。然而，需要研究PAGE工作人员接触丙烯酰胺是否存在遗传毒性和生殖影响的风险。非吸烟对照组中丙烯酰胺加合物的高背景是出乎意料的。这种背景的起源尚不清楚。丙烯腈加合物低于检测限（
Acrylamide is a chemical which is extensively used in research laboratories for the preparation of polyacrylamide gels for electrophoresis (PAGE). Blood samples were collected from laboratory personnel who were working with PAGE, from smokers, and from nonsmokers. Hemoglobin adducts of acrylamide, acrylonitrile, and ethylene oxide were determined using the modified Edman degradation procedure. Acrylamide adducts were detected in all persons. The PAGE workers (mean 54 pmol/g) had a significantly increased adduct level compared to nonsmoking controls (mean 31 pmol/g). The acrylamide adducts in smokers (mean 116 pmol/g) correlated with the number of cigarettes smoked per day. This confirms the presence of acrylamide in tobacco smoke and shows that it is an important source of acrylamide exposure. The increased level of acrylamide adducts in the PAGE workers corresponds to an uptake of acrylamide from about 3 cigarettes per day. It is not possible from this study to draw any conclusion as to which step in the working procedure is most critical for exposure. The PAGE workers are probably not at risk for neurotoxic damage to the peripheral nervous system. However, it needs to be investigated whether the exposure to acrylamide in PAGE workers represents a risk for genotoxic and reproductive effects. The high background of acrylamide adducts in nonsmoking controls was unexpected. The origin of this background is not known. Acrylonitrile adducts were below the detection limit (