The Hansenula polymorpha PER8 gene encodes a novel peroxisomal integral membrane protein involved in proliferation.
The Hansenula polymorpha PER8 gene encodes a novel peroxisomal integral membrane protein involved in proliferation.
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Hansenula Polymorpha PER8基因编码与增殖有关的新型过氧化物酶体积分膜蛋白。
DOI：
10.1083/jcb.128.3.307
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发表时间：
1995-02
影响因子：
7.8
通讯作者：
Cregg, J M
中科院分区：
文献类型：
--
作者：
Tan, X;Waterham, H R;Veenhuis, M;Cregg, J M
我们之前描述了过氧化物酶体生物发生缺陷的甲基营养酵母汉逊酵母突变体的分离。在这里，我们描述了这些突变体之一 per8 的特征，以及 PER8 基因的克隆。在甲醇或甲胺培养基（通常诱导细胞器的条件）中，per8 细胞不含过氧化物酶体样结构，并且过氧化物酶体酶位于细胞质中。 PER8的序列预测其产物(Per8p)是一种34kD的新多肽，并且针对Per8p的抗体识别31kD的蛋白质。对 Per8p 一级序列的分析揭示了一个 39 个氨基酸的富含半胱氨酸的片段，与锌指基序的 C3HC4 家族相似。 PER8 的过度表达导致过氧化物酶体数量显着增加。我们证明 Per8p 是过氧化物酶体的整合膜蛋白，并且它集中在新形成的细胞器的膜中。我们认为 Per8p 是控制该细胞器增殖的分子机制的一个组成部分。
We previously described the isolation of mutants of the methylotrophic yeast Hansenula polymorpha that are defective in peroxisome biogenesis. Here, we describe the characterization of one of these mutants, per8, and the cloning of the PER8 gene. In either methanol or methylamine medium, conditions that normally induce the organelles, per8 cells contain no peroxisome-like structures and peroxisomal enzymes are located in the cytosol. The sequence of PER8 predicts that its product (Per8p) is a novel polypeptide of 34 kD, and antibodies against Per8p recognize a protein of 31 kD. Analysis of the primary sequence of Per8p revealed a 39-amino-acid cysteine-rich segment with similarity to the C3HC4 family of zinc-finger motifs. Overexpression of PER8 results in a markedly enhanced increase in peroxisome numbers. We show that Per8p is an integral membrane protein of the peroxisome and that it is concentrated in the membranes of newly formed organelles. We propose that Per8p is a component of the molecular machinery that controls the proliferation of this organelle.