Changes in Human Meibum Lipid Composition with Age Using Nuclear Magnetic Resonance Spectroscopy
Changes in Human Meibum Lipid Composition with Age Using Nuclear Magnetic Resonance Spectroscopy
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DOI：
10.1167/iovs.11-8341
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发表时间：
2012-01-01
影响因子：
4.4
通讯作者：
Milliner, Sarah E.
中科院分区：
文献类型：
--
作者：
Borchman, Douglas;Foulks, Gary N.;Milliner, Sarah E.
目的。人类泪膜稳定性随着年龄的增长而降低。在这项研究中，测量了睑脂成分的变化，以寻找泪膜不稳定的标志物。方法。获得了43例1～88岁正常供体的H-1 NMR核磁共振(NMR)谱。结果。与青少年和成人的睑脂相比，婴儿和儿童的睑脂含有较少的CH3和C=C基团，并且醛与脂质过氧化氢的比率增加。结论。婴儿泪膜稳定性高于成人是合理的。它们的睑脂含有较少的 CH3 和 C=C 基团以及较高水平的蛋白质，因此，由于脂质-脂质相互作用更紧密和更强，脂质更加有序。为了使水蒸发，它必须首先穿过紧密的脂质屏障。为了使眼泪破裂，必须打破脂质与脂质之间的相互作用。这是合理的，因为与青少年和成人相比，婴儿和儿童泪液中的脂质相互作用更强，因此年轻群体的泪膜比成人的泪膜更稳定并且提供更好的蒸发屏障。脂质饱和可能是睑脂稳定婴儿眼泪的关键特征。 （投资眼科可见科学。2012 年；53：475-482）DOI：10.1167/iovs.11-8341
PURPOSE. Human tear film stability decreases with increasing age. In this study, the changes in meibum composition were measured in search of markers of tear film instability.METHODS. H-1 NMR nuclear magnetic resonance (NMR) spectra of 43 normal donors aged 1 to 88 years were acquired.RESULTS. Compared with meibum from adolescents and adults, meibum from infants and children contains less CH3 and C=C groups and an increased aldehyde-to-lipid hydroperoxide ratio.CONCLUSIONS. It is reasonable that tear film stability is higher in infants than in adults. Their meibum contains less CH3 and C=C groups and higher levels of protein, and as a result, the lipid is more ordered because of the tighter and stronger lipid-lipid interactions. For water to evaporate, it must first pass through the tight lipid-lipid barrier. For tears to break up, lipid-lipid interactions must be broken. It is reasonable that because the lipid-lipid interactions are stronger in infants' and children's tears compared with those of adolescents and adults, the tear film in the younger groups is more stable and provides a better barrier to evaporation than does the tear film of adults. Lipid saturation could be the critical feature in meibum that stabilizes tears in infants. (Invest Ophthalmol Vis Sci. 2012; 53: 475-482) DOI:10.1167/iovs.11-8341