Phylogenetic and expression analysis of lamprey toll-like receptors
Phylogenetic and expression analysis of lamprey toll-like receptors
复制标题
DOI：
10.1016/j.dci.2010.03.004
复制
发表时间：
2010-08-01
影响因子：
2.9
通讯作者：
Seya, Tsukasa
中科院分区：
文献类型：
--
作者：
Kasamatsu, Jun;Oshiumi, Hiroyuki;Seya, Tsukasa
Toll样受体（TLR）已被确定为脊椎动物中识别微生物模式分子的关键传感器。红鳍东方鲀的全基因组分析支持鱼类TLR基因的基本家族的存在。然而，先天免疫系统在提高无颌鱼获得性免疫力方面的作用仍不清楚。在本研究中，我们在同源性的基础上，从最新的七鳃鳗基因组组装预测的16七鳃鳗TLR基因进行了注释，并确定了它们的cDNA从日本七鳃鳗，Lethenteron japonicum。系统发育分析表明，七鳃鳗类TLR由鱼类（F）和哺乳动物（M）类TLR组成，并且七鳃鳗类TLR在不同器官中组成型表达。我们的研究结果表明，七鳃鳗保护免受微生物使用的先天系统组成的一组类似的M-和F-型TLR，尽管拥有独特的获得性免疫系统。此外，虽然TLR衔接子和信号转导基因在大多数脊椎动物中（IRF）-3/7和I型干扰素的上游高度保守，但在七鳃鳗基因组中未鉴定出I型干扰素（IFN）、干扰素调节因子（IRF）-3和IRF 7。这是第一份报告，描述TLR的剧目和IFN系统在最原始的脊椎动物之一，七鳃鳗。(C)2010爱思唯尔有限公司版权所有。
Toll-like receptors (TLRs) have been identified as pivotal sensors recognizing microbial pattern molecules in vertebrates. Whole genome analysis of the teleost Takifugu rubripes supports the existence of a fundamental family of TLR genes in fish. However, the role of the innate immune system in the context of raising acquired immunity in jawless fish remains unclear. In this study, we annotated 16 lamprey TLR genes predicted from the latest genome assembly of lamprey on the basis of homology, and identified their cDNAs from Japanese lamprey, Lethenteron japonicum. Phylogenetic analyses indicated that the repertoire of lamprey TLRs consisted of both fish (F)- and mammalian (M)-type TLRs, and it was also demonstrated that lamprey TLRs are constitutively expressed in various organs. Our results suggest that lampreys protect against microorganisms using the innate system consisting of a similar set of M- and F-type TLRs, despite possessing a unique acquired immune system. In addition, type I interferon (IFN), interferon regulatory factor (IRF)-3, and IRF7 were not identified in the lamprey genome although TLR adaptor and signal transduction genes were highly conserved upstream of (IRF)-3/7 and type I IFN in most vertebrates. This is the first report to describe the TLR repertoire and IFN system in one of the most primitive vertebrates, the lamprey. (C) 2010 Elsevier Ltd. All rights reserved.