Identification of miRNAs associated with tumorigenesis of retinoblastoma by miRNA microarray analysis
Identification of miRNAs associated with tumorigenesis of retinoblastoma by miRNA microarray analysis
复制标题
通过 miRNA 微阵列分析鉴定与视网膜母细胞瘤肿瘤发生相关的 miRNA
DOI：
10.1007/s00381-008-0701-x
复制
发表时间：
2009-01-01
影响因子：
1.4
通讯作者：
Ma, Xu
中科院分区：
文献类型：
--
作者：
Zhao, Jian-Jun;Yang, Juhua;Ma, Xu
microRNA（miRNAs）是一类小分子（α 1/222个核苷酸）调控RNA，在包括癌症在内的多种人类疾病中发挥重要作用。本研究采用microRNA芯片技术，通过北方印迹分析和原位杂交技术，对人视网膜母细胞瘤组织中差异表达的miRNAs进行了鉴定，筛选出一组在视网膜母细胞瘤中高表达的miRNAs，包括hsa-miR-494、hsa-let-7 e、hsa-miR-513-1、hsa-miR-513 - 2、hsa-miR-513- 3、hsa-miR-513- 4、hsa-miR-513- 5、hsa-miR-5 hsa-miR-513-2、hsa-miR-518 c *、hsa-miR-129-1、hsa-miR-129-2、hsa-miR-198、hsa-miR-492、hsa-miR-498、hsa-miR-320、hsa-miR-503和hsa-miR-373*。这些miRNAs是首次报道的人视网膜母细胞瘤的miRNAs，可能在调节肿瘤发生中发挥重要作用。
MicroRNAs (miRNAs) are small (a1/222 nucleotides) regulatory RNAs which play fundamental roles in many human diseases, including cancer. There is no report on the miRNA expression profile of retinoblastoma.This work was undertaken to identify differentially expressed miRNAs in human retinoblastoma tissues by microRNA microarray technique, and some miRNAs were verified using northern blot analysis and the in situ hybridization method.A cluster of microRNAs was identified as highly expressed in retinoblastoma, including hsa-miR-494, hsa-let-7e, hsa-miR-513-1, hsa-miR-513-2, hsa-miR-518c*, hsa-miR-129-1, hsa-miR-129-2, hsa-miR-198, hsa-miR-492, hsa-miR-498, hsa-miR-320, hsa-miR-503, and hsa-miR-373*.These miRNAs are the first to be reported for human retinoblastoma and may play significant roles in regulating tumor genesis.