LIGHT-SCATTERING AND ABSORPTION EFFECTS CAUSED BY ADDITION OF ADENOSINE DIPHOSPHATE TO RAT-HEART-MUSCLE SARCOSOMES
LIGHT-SCATTERING AND ABSORPTION EFFECTS CAUSED BY ADDITION OF ADENOSINE DIPHOSPHATE TO RAT-HEART-MUSCLE SARCOSOMES
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DOI：
10.1042/bj0680295
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发表时间：
1958-01-01
影响因子：
4.1
通讯作者：
PACKER, L
中科院分区：
文献类型：
--
作者：
CHANCE, B;PACKER, L
对心脏肌肉肌小体悬浮液中消光变化的同时单光束和双光束记录表明，添加二磷酸腺苷导致吸收的非特异性增加（在443 mμ处测量）以及在430 mμ处测量的吸收相对于443 mμ的特异性减少。非特定变化大约是特定变化的4倍。单光束分光光度技术不适用于测量同时显示光吸收非特异性变化的肌小体和线粒体悬浮液中的光谱效应。如果假设肌小体的非特异性光吸收特性指示离子传输过程，则这些结果表明氧化磷酸化过程中形成的中间体在防止肌小体肿胀方面比三磷酸腺苷本身更有效。
Simultaneous single-beam and double-beam recordings of extinction changes in a suspension of he art-muscle sarcosomes show that addition of adenosine diphosphate causes a non-specific increase of absorption (measured at 443 m[mu]) and a specific decrease of absorption at 430 m [mu] measured with respect to 443 m[mu]. The non-specific change is about 4 times as large as the specific one. A single-beam spectrophotometric technique is not suitable for the measurement of spectroscopic effects in suspensions of sarcosomes and mitochondria that show simultaneous non-specific changes of light-absorption. If it is assumed that the non-specific light-absorption properties of the sarcosomes are indicative of the ion-transport processes, these results suggest that intermediates formed during oxidative phosphorylation are more effective in preventing swelling of the sarcosomes than in adenosine triphosphate itself.