Human native soluble CD40L is a biologically active trimer, processed inside microsomes
Human native soluble CD40L is a biologically active trimer, processed inside microsomes
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DOI：
10.1074/jbc.271.11.5965
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发表时间：
1996-03-15
影响因子：
4.8
通讯作者：
Gauchat, JF
中科院分区：
文献类型：
--
作者：
Pietravalle, F;LecoanetHenchoz, S;Gauchat, JF
CD 40配体（CD 40 L）是一种糖蛋白，表达于活化的辅助性T细胞、嗜碱性粒细胞、肥大细胞和嗜酸性粒细胞的表面。CD 40 L与B细胞表面上其受体CD 40的结合诱导B细胞增殖、粘附和免疫球蛋白类别转换。我们已经确定了可溶性裂解产物的人CD 40 L的刺激的人T细胞克隆的上清液中。亚细胞分级实验表明，跨膜CD 40 L在微粒体内加工，其裂解是刺激依赖性的。天然人可溶性CD 40 L是三聚体，当与白细胞介素-4联合使用时，诱导B细胞增殖。
CD40 ligand (CD40L) is a glycoprotein expressed on the surface of activated helper T cells, basophils, mast cells, and eosinophils. Binding of CD40L to its receptor CD40 on the B cell surface induces B cell proliferation, adhesion, and immunoglobulin class switching. We have identified soluble cleavage products of human CD40L in the supernatant of a stimulated human T cell clone. Subcellular fractionation experiments have shown that the transmembrane CD40L is processed inside the microsomes and that its cleavage is stimulation-dependent. The native human soluble CD40L is trimeric and, when used in conjunction with interleukin-4, induces B cell proliferation.