Increased anesthesia time using 2,2,2-tribromoethanol-chloral hydrate with low impact on mouse psychoacoustics.
Increased anesthesia time using 2,2,2-tribromoethanol-chloral hydrate with low impact on mouse psychoacoustics.
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DOI：
10.1016/j.jneumeth.2013.07.004
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发表时间：
2013-09-30
影响因子：
3
通讯作者：
Gow, Alexander
中科院分区：
文献类型：
--
作者：
Maheras, Kathleen J.;Gow, Alexander
关键词：
To examine psychoacoustics in mice, we have used 2,2,2-tribromoethanol anesthesia in multiple studies. We find this drug is fast-acting and yields consistent results, providing 30 – 40 min of anesthesia. Our recent studies in binaural hearing prompted development of a regimen to anesthesia time to one hour. We tested a novel cocktail using 2,2,2-tribromoethanol coupled with low dose chloral hydrate to extend the effective anesthesia time. We have established an intraperitoneal dosing regimen for 2,2,2-tribromoethanol-chloral hydrate anesthesia. To measure efficacy of the drug cocktail, we measured auditory brainstem responses (ABRs) at 10 min intervals to determine the effects on hearing thresholds and wave amplitudes and latencies. This novel drug combination increases effective anesthesia to one hour. ABR Wave I amplitudes, but not latencies, are marginally suppressed. Additionally, amplitudes of the centrally-derived Waves III and V show significant inter-animal variability that is independent of stimulus intensity. These data argue against the systematic suppression of ABRs by the drug cocktail. Using 2,2,2-tribromoethanol-chloral hydrate combination in psychoacoustic studies has several advantages over other drug cocktails, the most important being preservation of latencies from centrally- and peripherally-derived ABR waves. In addition, hearing thresholds are unchanged and wave amplitudes are not systematically suppressed, although they exhibit greater variability. We demonstrate that 375 mg/kg 2,2,2-tribromoethanol followed after five min by 200 mg/kg chloral hydrate provides an anesthesia time of 60 min, has negligible effects on ABR wave latencies and thresholds and non-systematic effects on amplitudes.
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DOI：
10.1016/b978-012373898-1.50014-0
发表时间：
2008-01-01
期刊：
ANESTHESIA AND ANALGESIA IN LABORATORY ANIMALS, 2ND EDITION
影响因子：
--
作者：
Gaertner, Diane J.;Hallman, Troy M.;Batchelder, Margaret A.
通讯作者：
Batchelder, Margaret A.
影响因子：
3.6
作者：
HETZLER, BE;DYER, RS
通讯作者：
DYER, RS
影响因子：
64.5
作者：
Gow, A;Southwood, CM;Lazzarini, RA
通讯作者：
Lazzarini, RA
影响因子：
2.8
作者：
Zheng, QY;Johnson, KR;Erway, LC
通讯作者：
Erway, LC
影响因子：
2.5
作者：
MCGEE, TJ;OZDAMAR, O;KRAUS, N
通讯作者：
KRAUS, N