Sphingosinicella microcystinivorans gen. nov., sp nov., a microcystin-degrading bacterium
Sphingosinicella microcystinivorans gen. nov., sp nov., a microcystin-degrading bacterium
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DOI：
10.1099/ijs.0.63789-0
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发表时间：
2006-01-01
影响因子：
2.8
通讯作者：
Kato, K
中科院分区：
文献类型：
--
作者：
Maruyama, T;Park, HD;Kato, K
从日本的富营养化湖泊-这些菌株是需氧和化学有机营养型的，它们的细胞是革兰氏阴性的，不形成孢子的杆状，通过单极鞭毛运动。营养琼脂培养基上形成黄色菌落。在供试的有机碳源中，菌株仅同化柠檬酸盐。基因组DNA的G + C含量为63.6 ~ 63.7mol%。基于16 S rRNA基因序列的系统发育分析表明，新的分离物形成了一个紧密的簇内的家庭鞘氨醇单胞菌科，但显然是独立的已建立的属，这个家庭，如鞘氨醇单胞菌，鞘氨醇，Novosphingobium，和Sphingopyxis;新的分离物和模式菌株之间的序列相似性从90.9%到94.9%。化学分类和表型数据支持的结论，这些菌株的家庭鞘氨醇单胞菌科的成员。细胞脂肪酸的主要成分是18：1 omega 7 c（36-41%）和16：1 omega 7 c（33-36%）。羟基脂肪酸主要为2-OH 14：0（11-13%），不含3-OH脂肪酸。检测到鞘糖脂。泛醌-10和homospermidine分别作为主要的奎宁和多胺。因此，建议这三个菌株代表鞘氨醇单胞菌科的一个新属和新种，命名为Sphingosinicella microcystinivorans gen. nov.，模式菌株为Y2（T）（= KCTC; 12019（T）=JCM 13185（T））。
Three strains of bacteria that degrade the cyanobacterial hepatotoxin microcystin, Y2(T), MDB2 and MDB3, were isolated from a eutrophic lake, Lake Suwa, and the Tenryu River, Japan, and characterized. These strains were aerobic and chemo-organotrophic and their cells were Gram-negative, non-spore-forming rods, motile by means of single polar flagella. Yellow-pigmented colonies were formed on nutrient agar media. The strains assimilated only citrate among the organic compounds tested as carbon sources. The G + C content of genomic DNA ranged from 63.6 to 63.7 mol%. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the new isolates formed a tight cluster within the family Sphingomonadaceae but were clearly separate from established genera of this family, e.g. Sphingomonas, Sphingobium, Novosphingobium, and Sphingopyxis; sequence similarities between the new isolates and type strains from established genera ranged from 90.9 to 94.9%. Chemotaxonomic and phenotypic data supported the conclusion that these strains were members of the family Sphingomonadaceae. The major components of the cellular fatty acids were 18:1 omega 7c (36-41%) and 16:1 omega 7c (33-36%). Hydroxy fatty acids were mainly 2-OH 14: 0 (11-13%), and 3-OH fatty acids were absent. Glycosphingolipids were detected. Ubiquinone-10 and homospermidine were present as the major quinine and polyamine, respectively. Thus, it is proposed that the three strains represent a new genus and species of the family Sphingomonadaceae with the name Sphingosinicella microcystinivorans gen. nov., sp. nov. The type strain is Y2(T) (= KCTC; 12019(T)=JCM 13185(T)).