Conception Rate and Reproductive Hormone Secretion in Holstein Cows Immunized against Inhibin and Subjected to the Ovsynch Protocol
Conception Rate and Reproductive Hormone Secretion in Holstein Cows Immunized against Inhibin and Subjected to the Ovsynch Protocol
复制标题
免疫抑制素并接受 Ovsynch 方案的荷斯坦奶牛的受孕率和生殖激素分泌
DOI：
10.3390/ani10020313
复制
发表时间：
2020-02-01
期刊：
影响因子：
3
通讯作者：
Shi, Zhendan
中科院分区：
文献类型：
--
作者：
Guo, Rihong;Chen, Fang;Shi, Zhendan
近几十年来，泌乳奶牛的受胎率急剧下降，提高受胎率已成为奶牛繁殖的主要挑战。已经开发了各种Ovsynch方案来帮助恢复卵巢卵泡活动，以便产后奶牛及时繁殖。然而，Ovsynch协议在提高受胎率方面的效果并不令人满意。此外，据报告，对阿托宾进行免疫接种可提高家畜的生育力。因此，本研究提出并测试了一种新的生殖技术，该技术结合了针对Escherichbin的免疫接种和广泛使用的Ovsynch方案。我们的研究结果表明，免疫接种抗生育激素有可能提高奶牛的受孕率，但也损害了黄体功能。根据这些结果，建议在免疫和Ovsynch方案的基础上进行额外的促黄体治疗，以进一步提高奶牛生育力。摘要本研究旨在探讨通过对奶牛免疫来提高奶牛繁殖力的可行性。将32头奶牛分为对照组（n = 11）、低剂量组（n = 10）和高剂量组（n = 11）。高剂量组和低剂量组奶牛分别接受1 mg和0.5 mg Escherichin免疫原给药。所有奶牛从抗原给药当天开始接受Ovsynch方案，并进行人工授精。从Ovsynch方案开始到授精后14天，在24天内连续采集血样。结果表明，免疫抗卵泡刺激素（FSH），雌二醇（E2），激活素A的血浆浓度呈剂量依赖性增加，但降低孕酮（P4）浓度在黄体期。在初次授精后第14天，免疫还增加了妊娠母牛的血浆干扰素（IFN）-τ浓度。高剂量组（45.5%）和低剂量组（40%）的受胎率与对照组（27.3%）相比略有增加，但增加不显著（p > 0.05）。总之，一个单一的免疫接种，对阿托宾有可能提高受孕率，尽管受损的黄体发育。为了进一步提高奶牛的繁殖性能，建议结合针对Escherichin和Ovsynch技术的免疫接种进行额外的黄体刺激治疗。
Simple Summary In recent decades, conception rates in lactating dairy cows have dramatically decreased, and improving the conception rate has become a major challenge in dairy cow reproduction. Various Ovsynch protocols have been developed to aid in the resumption of ovarian follicular activity for timely breeding in post-partum cows. However, the effect of Ovsynch protocols on improving the conception rate is unsatisfactory. In addition, immunization against inhibin was reported to improve the fertility of domestic animals. Thus, a novel reproductive technique combining immunization against inhibin and the widely used Ovsynch protocol was proposed and tested in this study. Our results showed that immunization against inhibin has the potential to improve conception rates in cows, but also compromised luteal function. According to these results, additional luteal-stimulating treatments are suggested to further improve cow fertility based on immunization and the Ovsynch protocol. Abstract This study was conducted to investigate the feasibility of improving fertility in dairy cows via immunization against inhibin. Thirty-two cows were divided into Control (n = 11), Low-dose (n = 10) and High-dose (n = 11) groups. The High-dose and Low-dose cows were treated with 1 and 0.5 mg of the inhibin immunogen, respectively. All the cows were subjected to the Ovsynch protocol from the day of antigen administration and were artificially inseminated. Blood samples were serially collected over a 24-day period from the start of the Ovsynch protocol to 14 days after insemination. The results showed that immunization against inhibin dose-dependently increased the plasma concentrations of follicle-stimulating hormone (FSH), estradiol (E2), and activin A, but decreased progesterone (P4) concentrations in the luteal phase. Immunization also increased the plasma interferon (IFN)-τ concentrations in pregnant cows on day 14 after initial insemination. The conception rates in High-dose (45.5%) and Low-dose (40%) cows marginally increased compared to that in Control cows (27.3%), but the increases were not significant (p > 0.05). In conclusion, a single immunization against inhibin has the potential to improve conception rates, despite impaired luteal development. To further improve the reproductive performance of dairy cows, additional luteal-stimulating treatments are suggested in combination with immunization against inhibin and Ovsynch techniques.