Partial protection against four species of chicken coccidia induced by multivalent subunit vaccine
Partial protection against four species of chicken coccidia induced by multivalent subunit vaccine
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多价亚单位疫苗对四种鸡球虫的部分保护作用
DOI：
10.1016/j.vetpar.2015.08.026
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发表时间：
2015-09-15
影响因子：
2.6
通讯作者：
Li, Xiangrui
中科院分区：
文献类型：
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作者：
Song, Xiaokai;Gao, Yunlu;Li, Xiangrui
在这项研究中，设计了一种多价亚单位疫苗来保护鸡免受几种艾美耳球虫同时感染。该疫苗含有来自四种艾美耳球虫（E. tenella、E. necatrix、E. acervulina 和 E. maxima）的重组蛋白，并在动物中进行了功效评估。为了生产这种疫苗，首先通过肌内接种和随后的攻击在鸡中筛选来自每个艾美耳球虫物种的候选抗原。测试的抗原包括来自柔嫩艾美球虫的重组蛋白 TA4 和 507、来自恶毒艾美球虫的 NA4 和 NPmz19、来自牛艾美球虫的 LDH、3-1E 和 MIF，以及来自巨型艾美球虫的 Em6 和 Em8（EmTFP250 的两个部分）。然后进行同源攻击以确定来自每个物种的哪种抗原提供最好的保护。被确定为其物种最具保护性的抗原随后受到异源物种的攻击。最后，将来自四个不同物种的重组蛋白以终浓度400μg/ml（每种蛋白100μg/ml）混合，形成多价亚单位疫苗，并在动物中测试其功效。结果表明，来自柔嫩艾美球虫的 TA4、来自恶毒艾美球虫的 NA4、来自牛艾美球虫的 LDH 和来自巨型艾美球虫的 Em8 各自诱导了针对同源攻击的最有效的保护。交叉保护结果表明，TA4对柔嫩艾美耳球虫提供部分交叉保护，NA4对柔嫩艾美耳球虫和牛艾美耳球虫提供部分交叉保护，LDH对柔嫩艾美耳球虫和柔嫩艾美耳球虫提供部分交叉保护，Em8对柔嫩艾美耳球虫和柔美艾美耳球虫提供部分交叉保护。多价亚单位疫苗针对柔嫩艾美耳球虫、恶毒艾美耳球虫、弯形艾美耳球虫和巨型艾美耳球虫的攻击提供了部分保护，并导致 ACI 超过 170。这些结果表明，我们的候选多价疫苗可以保护鸡免受多种艾美耳球虫同时感染。 (C) 2015 Elsevier B.V. 保留所有权利。
In this study, a multivalent subunit vaccine was designed to protect chickens against simultaneous infection by several Eimeria species. This vaccine contains recombinant proteins from four Eimeria species - E. tenella, E. necatrix, E. acervulina and E. maxima - and was evaluated for efficacy in animals. To produce this vaccine, candidate antigens from each Eimeria species were first screened in chickens via intramuscular inoculation and subsequent challenge. Antigens tested include recombinant proteins TA4 and 507 from E. tenella, NA4 and NPmz19 from E. necatrix, LDH, 3-1E and MIF from E. acervulina, and Em6 and Em8 (two portions of EmTFP250) from E. maxima. A homologous challenge was then performed to identify which antigen from each species conferred the best protection. The antigens identified as most protective against its species were then challenged by heterologous species. Finally, the selected recombinant proteins from each of the four respective species were mixed with the final concentration of 400 mu g/ml (100 mu g of each protein/ml) to form the multivalent subunit vaccine, which was tested for efficacy in animals. The results indicated that TA4 from E. tenella, NA4 from E. necatrix, LDH from E. acervulina, and Em8 from E. maxima each induced the most effective protection from homologous challenge. Cross-protection results showed that TA4 provided partial cross-protection against E. necatrix, NA4 provided partial cross-protection against E. tenella and E. acervulina, LDH provided partial cross-protection against E. tenella and E. necatrix, and Em8 provided partial cross-protection against E. tenella and E. acervulina. The multivalent subunit vaccine provided partial protection against E. tenella, E. necatrix, E. acervulina and E. maxima challenge, and resulted in ACIs of more than 170. These results suggest that our candidate multivalent vaccine could protect chickens against simultaneous infection by several Eimeria species. (C) 2015 Elsevier B.V. All rights reserved.