A BRIEF PHOTOCHEMICALLY INDUCED OXIDATIVE INSULT CAUSES IRREVERSIBLE LENS DAMAGE AND CATARACT .1. TRANSPARENCY AND EPITHELIAL-CELL LAYER
A BRIEF PHOTOCHEMICALLY INDUCED OXIDATIVE INSULT CAUSES IRREVERSIBLE LENS DAMAGE AND CATARACT .1. TRANSPARENCY AND EPITHELIAL-CELL LAYER
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DOI：
10.1016/s0014-4835(05)80062-4
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发表时间：
1995-05-01
影响因子：
3.4
通讯作者：
KUSZAK, JR
中科院分区：
文献类型：
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作者：
SPECTOR, A;WANG, GM;KUSZAK, JR
K_2O_2、O-_2（.-）对培养大鼠晶状体的短期光化学损伤哦。发现导致对上皮细胞层的快速不可逆损伤。通过台盼蓝染色、末端脱氧核糖核苷酸转移酶标记、DNA梯状条带和形态学分析来测量这种不可逆的损伤。光化学损伤的时间和观察上皮细胞损伤所需的损伤后时间之间似乎存在反比关系。几个小时的损伤期需要数天的损伤后间隔来观察明显的细胞损伤，在发现完全的皮质性白内障之前需要数周。
Short-term photochemical insult of cultured rat lenses caused by the generation of K2O2, O-2(.-) and OH. was found to lead to rapid irreversible damage to the epithelial cell layer. This irreversible damage was measured by Trypan blue staining, terminal deoxyribonucleotidyl transferase labeling, DNA laddering and morphological analyses. There appears to be an inverse relationship between the period of photochemical insult and the post-insult time required to observe epithelial cell damage. Insulting periods of a few hours require post-insult intervals of days to observe significant cell damage and weeks before complete cortical cataracts are found. Epithelial cell damage precedes the loss of transparency.