Establishment of passive hemagglutination assay (PHA) system for anti-HBc in plasma.
Establishment of passive hemagglutination assay (PHA) system for anti-HBc in plasma.
复制标题
血浆抗-HBc被动血凝测定（PHA）系统的建立。
DOI：
10.1620/tjem.149.11
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发表时间：
1986
影响因子：
2.2
通讯作者：
H. Ohori
中科院分区：
文献类型：
--
作者：
Y. Uemura;K. Fukuyama;M. Nishida;T. Suyama;H. Ohori
建立了一种灵敏、特异的抗hbc检测系统——被动血凝（PHA）法。纯化重组hbcg -颗粒与固定羊血细胞（SRBC）偶联制备的PHA细胞对单克隆和多克隆抗hbc igg具有高度特异性。PHA法的灵敏度高于放射免疫分析法（RIA）。然而，血浆中抗hbc阳性的不确定性仍然存在，PHA滴度低于2(5)。在血液被认为适合输血的献血者中，被证实为HBsAg阴性的抗HBsAg阳性血浆比例相对较高（19%，196人中有37人）。本文讨论了抗hbc阳性血液的危害及血浆中抗hbc检测的重要性。
A sensitive and specific assay system for anti-HBc, the passive hemagglutination (PHA) method, has been established. The reactivity of PHA cells prepared by conjugating purified recombinant HBcAg-particles with fixed sheep blood cells (SRBC) was highly specific to monoclonal- and polyclonal anti-HBc IgGs. The sensitivity of PHA method was higher than that of radioimmunoassay (RIA). However, uncertainty for the positivity of anti-HBc still remained in plasma with the PHA titers lower than 2(5). A relatively high ratio (19%, 37 of 196) of anti-HBc-positive plasma, which had been confirmed to be HBsAg negative, was demonstrated in blood donors whose bloods have been considered suitable for transfusion. The hazards of anti-HBc-positive bloods and the importance of anti-HBc detection in plasma are discussed in this paper.