pRB and p107/p130 are required for the regulated expression of different sets of E2F responsive genes
pRB and p107/p130 are required for the regulated expression of different sets of E2F responsive genes
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DOI：
10.1101/gad.11.11.1447
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发表时间：
1997-06-01
影响因子：
10.5
通讯作者：
Dyson, N
中科院分区：
文献类型：
--
作者：
Hurford, RK;Cobrinik, D;Dyson, N
E2 F转录因子的活性由与视网膜母细胞瘤蛋白（pRB）和两种相关蛋白p107和p130的物理结合控制。pRB家族成员被认为控制E2 F活性的不同方面，但目前还不清楚这些蛋白质各自的功能可能是什么。为了剖析pRB、p107和p130的特定功能，我们研究了E2 F调节基因的表达在缺乏这些家族成员的原代细胞培养物中是如何改变的。而没有变化的E2 F-靶基因的表达在细胞中缺乏p107或p130，失调的E2 F目标的表达被认为是在细胞缺乏pRB和细胞缺乏p107和p130。令人惊讶的是，在这两种情况下失调的基因是完全不同的。这些发现表明PRE和p107/p130确实在E2 F调节中提供了不同的功能，并鉴定了依赖于pRB家族蛋白正常表达的靶基因。
The activity of the E2F transcription factor is controlled by physical association with the retinoblastoma protein (pRB) and two related proteins, p107 and p130. The pRB family members are thought to control different aspects of E2F activity, but it has been unclear what the respective functions of these proteins might be. To dissect the specific functions of pRB, p107, and p130 we have investigated how the expression of E2F-regulated genes is changed in cultures of primary cells lacking each of these family members. Whereas no changes were found in the expression of E2F-target genes in cells lacking either p107 or p130, deregulated expression of E2F targets was seen in cells lacking pRB and in cells lacking both p107 and p130. Surprisingly, the genes that were disregulated in these two settings were completely different. These findings show that PRE and p107/p130 indeed provide different functions in E2F regulation and identify target genes that are dependent on pRB family proteins for their normal expression.