RNA interference against repulsive guidance molecule A improves axon sprout and neural function recovery of rats after MCAO/reperfusion
RNA interference against repulsive guidance molecule A improves axon sprout and neural function recovery of rats after MCAO/reperfusion
复制标题
RNA干扰排斥导向分子A可改善MCAO/再灌注后大鼠的轴突萌芽和神经功能恢复
DOI：
10.1016/j.expneurol.2012.08.014
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发表时间：
2012-12-01
影响因子：
5.3
通讯作者：
Qin, Xinyue
中科院分区：
文献类型：
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作者：
Feng, Jinzhou;Wang, Tianzhu;Qin, Xinyue
排斥导向分子a（RGMa）是一种神经突起生长抑制剂，在中枢神经系统神经元再生的研究中具有重要意义。我们采用RNA干扰（RNAi）的方法抑制RGMa的表达，观察其对脑缺血损伤后大鼠轴突再生和神经功能的改善作用。重组腺病毒rAd 5-shRNA-RGMa的构建和制备用于动物实验。采用逆转录聚合酶链反应（RT-PCR）和免疫组织化学方法检测缺血侧皮质和海马RGMa和神经丝蛋白200（NE 200）的表达。氯化三苯基四氮唑（TTC）染色观察缺血区，生物素葡聚糖胺（BDA）神经元示踪观察新生神经突起分支。采用行为学检查评价神经功能恢复情况。结果显示，RNAi组RGMa表达下调，轴突生长明显改善（P
Repulsive guidance molecule a (RGMa) is a neurite growth inhibitor that is of great interest in the study of CNS neuronal regeneration. We adopted RNA interference (RNAi) as a means of suppressing the expression of RGMa and observed the improvement in axonal regeneration and neurological function of rats after cerebral ischemic injury. Recombinant adenovirus rAd5-shRNA-RGMa was constructed and prepared for animal experimentation. RGMa and neurofilament protein 200 (NE200) in the ischemic cortex and ipsilateral hippocampus were detected by reverse transcription polymerase chain reaction (RT-PCR) and immunohistochemistry. The ischemic regions were examined by triphenyltetrazolium chloride (TTC) staining and the newborn neurite branches by Biotinylated Dextran Amine (BDA) neuronal tracing. Behavior tests were adopted to evaluate neurologic function recovery. Results showed RGMa was down-regulated and axonal growth was improved in the RNAi treated group (P